Direct visualization of IgM antibodies bound to tissue antigens using a monoclonal anti-type III collagen IgM as a model system.
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Summary
The data suggest that direct visualization of primary monoclonal IgM antibodies may be useful in a wide variety of highly specific ultrastructural immunolocalization studies without requiring the use of electron-dense conjugates.
- Type
- article
- Published
- 1987-03-01
- Cited by
- 19
- References
- 24
- Access
- Open access
- OpenAlex
- https://openalex.org/W2013538768
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:33108779
Keywords
Monoclonal antibody, Antibody, Chemistry, Conjugate, Primary and secondary antibodies
References
- The use of ferritin-conjugated antibodies in electron microscopy.
- Immunoelectron microscopy of type III collagen in normal and scleroderma skin.
- ENZYME-LABELED ANTIBODIES: PREPARATION AND APPLICATION FOR THE LOCALIZATION OF ANTIGENS
- An immunocolloid method for the electron microscope.
- Large complex globular domains of type VII procollagen contribute to the structure of anchoring fibrils.
- Collagen fibrillogenesis in vitro: a characterization of fibril quality as a function of assembly conditions.
- Preparation of an Electron-dense Antibody Conjugate
- Protein A-peroxidase: a valluable tool for the localization of antigens.
- Fetal membrane collagens: identification of two new collagen alpha chains.
- Structure of hydrated immunoglobulins and antigen-antibody complexes. Electron microscopy of spray-freeze-etched specimens.
- THE THREE‐DIMENSIONAL CONFORMATION OF γ M and γ A GLOBULIN MOLECULES
- THE PROPERTIES OF SPECIFIC STAINS FOR ELECTRON MICROSCOPY PREPARED BY THE CONJUGATION OF ANTIBODY MOLECULES WITH FERRITIN
- Conformation of the Free and Antigen-bound IgM Antibody Molecules
- Staphylococcal Protein A in Immunoferritin Techniques
- Colloidal gold : a cytochemical marker for light and fluorescent microscopy and for transmission and scanning electron microscopy.
- Electron microscopy of the collagen fibril
Cited by
- Immunochemical analysis of human kidney reticulin.
- Collagen fibrillogenesis in vitro: interaction of types I and V collagen regulates fibril diameter.
- D-periodic distribution of collagen type IX along cartilage fibrils
- Monoclonal antibodies that distinguish avian type I and type III collagens: isolation, characterization and immunolocalization in various tissues.
- Immunochemistry of a keratinocyte-fibroblast co-culture model for reconstruction of human skin.
- Dermal collagen fibrils are hybrids of type I and type III collagen molecules.
- Human bone contains type III collagen, type VI collagen, and fibrillin: type III collagen is present on specific fibers that may mediate attachment of tendons, ligaments, and periosteum to calcified bone cortex.
- Direct visualization of an IgM directed against a membrane antigen involved in both cell‐matrix interactions and microfilament organization
- Preparation and applications of monoclonal antibodies to different collagen types.
- Type I and Type III Collagen Interactions during Fibrillogenesis a
- Type III collagen can be present on banded collagen fibrils regardless of fibril diameter
- Fourier analysis of electron micrographs of positively stained collagen fibrils: application to type I and II collagen typing.
- Acquisition of type IX collagen by the developing avian primary corneal stroma and vitreous.
- Ultrastructure of type VI collagen in human skin and cartilage suggests an anchoring function for this filamentous network
- Extraction of extendable beaded structures and their identification as fibrillin-containing extracellular matrix microfibrils.
- Type V collagen: molecular structure and fibrillar organization of the chicken alpha 1(V) NH2-terminal domain, a putative regulator of corneal fibrillogenesis
- The evolution of fat grafting: from plastic surgery to regenerative medicine
- Collagen Formation and Structure
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