A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts.
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Summary
With this method 1 ng of active enzyme can easily be detected and both prokaryotic and eukaryotic cell extracts can be examined, and changes in the size of enzymatically active proteins can be determined.
- Type
- article
- Published
- 1984-10-01
- Cited by
- 371
- References
- 17
- OpenAlex
- https://openalex.org/W2012019347
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:40440592
Keywords
Kanamycin, Biology, Neomycin, Biochemistry, Enzyme
References
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- Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
- Plasmid-determined resistance to antimicrobial agents.
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
- Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
- Specific interaction between enhancer-containing molecules and cellular components.
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- Transformation of CW-15 Mutant Cells of Chlamydomonas reinhardtii Dang. with pCTVHyg Plasmid
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- Plant Regeneration and Agrobacterium-Mediated Gene Transfer Studies in Leaf Tissue of male Himalayan Poplar (Populus ciliata Wall.)
- Biotechnology applications of Populus micropropagation
- Selectable markers for rice transformation.
- Preparation and transformation of monocot protoplasts.
- The Transformation of the Unicellular Alga Chlamydomonas reinhardtii by Electroporation
- Gene transfer into hematopoietic progenitor cells from normal and cyclic hematopoietic dogs using retroviral vectors.
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