Rapid evolution of a protein in vitro by DNA shuffling
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Summary
It is reported here that selected mutants had a minimum inhibitory concentration of 640 μg ml-1, a 32,000-fold increase and 64-fold greater than any published TEM-1 derived enzyme.
- Type
- article
- Published
- 1994-08-04
- Cited by
- 1,950
- References
- 20
- OpenAlex
- https://openalex.org/W2011513747
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:4363498
Keywords
DNA shuffling, Shuffling, Mutagenesis, Homologous recombination, Directed evolution
References
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- Phage antibodies: filamentous phage displaying antibody variable domains
- Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme.
- Directed molecular evolution.
- Systems: An Introductory Analysis with Applications to Biology, Control, and Artificial Intelligence, by John H. Holland MIT Press (Bradford Books), Cambridge, Mass., 1992, xiv+211 pp. (Paperback £13.50, cloth £26.95)
- An algorithm for protein engineering: simulations of recursive ensemble mutagenesis.
- More extended-spectrum beta-lactamases
- A new revision of the sequence of plasmid pBR322.
- The origins of order
- Extended-spectrum beta-lactamases
- Randomization of genes by PCR mutagenesis.
- Genetic Algorithms
- The Origins of Order
Cited by
- Pharmaceutical and industrial protein engineering: where we are?
- High-fidelity in vitro recombination using a proofreading polymerase.
- Staphylococcal surface display in directed evolution
- A DNA implementation of the Max 1s problem
- Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences.
- Isolation of Erythropoietin Receptor Agonist Peptides Using Evolved Phage Libraries
- PERSPECTIVE: SEX, RECOMBINATION, AND THE EFFICACY OF SELECTION—WAS WEISMANN RIGHT?
- Designed evolution of enzymatic properties.
- Exploring the functional robustness of an enzyme by in vitro evolution.
- Biotechnology and bioremediation: successes and limitations
- Modification of post-PKS tailoring steps through combinatorial biosynthesis.
- Protein engineering 20 years on
- Protein evolution by "codon shuffling": a novel method for generating highly variant mutant libraries by assembly of hexamer DNA duplexes.
- Predicting oligonucleotide-directed mutagenesis failures in protein engineering.
- Investigation of the electrochemical activity of chromium tolerant mutants of Geobacter metallireducens
- In vitro evolution of proteins.
- Enhancement of the activity and alkaline pH stability of Thermobifida fusca xylanase A by directed evolution
- Catalytic DNAzymes: derivations and functions
- E factors, green chemistry and catalysis: an odyssey.
- Directed evolution of chemotaxis inhibitory protein of Staphylococcus aureus generates biologically functional variants with reduced interaction with human antibodies.
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