Enzymatic mutation detection. Phosphate ions increase incision efficiency of endonuclease VII at a variety of damage sites in DNA.

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Summary

Improved reaction conditions are reported which can increase the selectivity of the enzyme for mismatches up to 500-fold, as demonstrated with a mutation in a 247 nt long fragment from exon 7 of human gene p53.

Type
article
Published
1998-05-01
Cited by
12
References
11

Keywords

Cleave, Heteroduplex, Endonuclease, Cleavage (geology), DNA

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