Enzymatic mutation detection. Phosphate ions increase incision efficiency of endonuclease VII at a variety of damage sites in DNA.
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Summary
Improved reaction conditions are reported which can increase the selectivity of the enzyme for mismatches up to 500-fold, as demonstrated with a mutation in a 247 nt long fragment from exon 7 of human gene p53.
- Type
- article
- Published
- 1998-05-01
- Cited by
- 12
- References
- 11
- OpenAlex
- https://openalex.org/W2001199763
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:20735999
Keywords
Cleave, Heteroduplex, Endonuclease, Cleavage (geology), DNA
References
- Studies on T4-head maturation. 1. Purification and characterization of gene-49-controlled endonuclease.
- The locus of sequence-directed and protein-induced DNA bending
- Model for the interaction of DNA junctions and resolving enzymes.
- Calibration of DNA curvature and a unified description of sequence-directed bending.
- Endonuclease VII of phage T4 triggers mismatch correction in vitro.
- Cruciform-resolvase interactions in supercoiled DNA.
- Studies on T4-head maturation. 2. Substrate specificity of gene-49-controlled endonuclease.
- T4 endonuclease VII resolves cruciform DNA with nick and counter-nick and its activity is directed by local nucleotide sequence.
- Detection of 81 of 81 known mouse beta-globin promoter mutations with T4 endonuclease VII--the EMC method.
- Improved large-scale preparation of phage T4 endonuclease VII overexpressed in E. coli.
- On the role of DNA replication, endonuclease VII, and rII proteins in processing of recombinational intermediates in phage T4.
Cited by
- Automated mutation analysis
- Cleavage of mispaired heteroduplex DNA substrates by numerous restriction enzymes.
- Specific binding of poly(ADP-ribose) polymerase-1 to cruciform hairpins.
- Enzymatic mutation detection: enrichment of heteroduplexes from hybrid DNA mixtures by cleavage-deficient GST-tagged endonuclease VII.
- Enzymatic mutation detection method evaluated for detection of p53 mutations in cDNA from breast cancers.
- Carbohydrate-deficient transferrin measured by capillary zone electrophoresis and by turbidimetric immunoassay for identification of young heavy drinkers.
- The restriction site mutation assay: a review of the methodology development and the current status of the technique.
- Combination of His-tagged T4 endonuclease VII with microplate array diagonal gel electrophoresis for high-throughput mutation scanning.
- Sequence variation in genes and genomic DNA: methods for large-scale analysis.
- Zur Differenzierung hereditärer sensomotorischer Neuropathien mittels Mutationsanalyse des P0-Gens an Paraffin-eingebetteten Suralnervenbiopsien
- Enzymatic mutation detection: enrichment of heteroduplexes from hybrid DNA mixtures by cleavage-deficient GST-tagged endonuclease VII
- CHAPTER 9 DNA HYBRIDIZATION IN SOLUTION FOR MUTATION DETECTION
- DNA Hybridization in Solution for Mutation Detection
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