PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database.
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Summary
PRIMROSE is a computer program for identifying 16S rRNA probes and PCR primers for use as phylogenetic and ecological tools in the identification and enumeration of bacteria and a comparison with PROBE DESIGN within the ARB software package shows that PRIMRose is capable of identifying oligonucleotides with a higher specificity.
- Type
- article
- Published
- 2002-08-01
- Cited by
- 258
- References
- 25
- Access
- Open access
- OpenAlex
- https://openalex.org/W1999195817
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:16973643
Keywords
Biology, Oligonucleotide, Computational biology, Ribosomal RNA, Phylogenetic tree
References
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- In Situ Analysis of Sulfate-Reducing Bacteria Related to Desulfocapsa thiozymogenes in the Chemocline of Meromictic Lake Cadagno (Switzerland)
- Phylogenetic Characterization and In Situ Detection of a Cytophaga-Flexibacter-Bacteroides Phylogroup Bacterium in Tuber borchii Vittad. Ectomycorrhizal Mycelium
Cited by
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- Kinetic parameters for nutrient enhanced crude oil biodegradation in intertidal marine sediments
- Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analysed by flow cytometry.
- Impact of endophytic colonization patterns on Zamioculcas zamiifolia stress response and in regulating ROS, tryptophan and IAA levels under airborne formaldehyde and formaldehyde-contaminated soil conditions.
- Caractérisation des communautés procaryotiques impliquées dans la bioremédiation d'un sol pollué par des hydrocarbures et développement d'outils d'analyse à haut débit : les biopuces ADN
- The Association of Outer Ear Commensal Bacteria with Otitis Media in Children
- Bistro-Primer - Tool to design and validate specific PCR primer pairs for phylogenetic analysis
- The Physiological and Ecological Characterisation of the First Cultivated Species of the Candidate Division OP10
- High-Throughput Analysis of the Intestinal Microbiota in Clostridium Difficile-Associated Disease
- Studies on the acorn barnacle Balanus amphitrite and its associated bacteria
- The First Insights into the Phylogeny, Genomics, and Ecology of the Novel Bacterial Phylum Armatimonadetes
- Algorithms and Software for PCR Primer Design
- Detecting unknown sequences with DNA microarrays: explorative probe design strategies.
- An investigation into the potential impacts of ocean acidification and ocean fertilisation on the genetic diversity of marine bacterial assemblages
- Aerobe Deproteinierung von Crustaceaen-Abfällen zur Gewinnung von Chitin mittels proteolytischer Mikroorganismen
- Ribosomal tag pyrosequencing of DNA and RNA from benthic coral reef microbiota: community spatial structure, rare members and nitrogen-cycling guilds.
- probeCheck – a central resource for evaluating oligonucleotide probe coverage and specificity
- Non-target sites with single nucleotide insertions or deletions are frequently found in 16S rRNA sequences and can lead to false positives in fluorescence in situ hybridization (FISH).
- Identification of signature and primers specific to genus Pseudomonas using mismatched patterns of 16S rDNA sequences
- Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae.
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