Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics.
Explore this paper's citation graph
Summary
The hypoosmotic swelling technique to evaluate the functional integrity of the sperm membrane appears to give high repeatability and accuracy and is closely correlated to the in-vitro fertilizing ability of spermatozoa.
- Type
- article
- Published
- 1984-01-01
- Cited by
- 1,827
- References
- 18
- Access
- Open access
- OpenAlex
- https://openalex.org/W1999099572
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:21001724
Keywords
Semen, Sperm, Andrology, Swelling, Biology
References
- Penetration of the zona-free hamster egg by human sperm.
- Sperm morphology assessment as an indicator of human fertilizing capacity.
- Sizing of aging bull spermatozoa with an electronic counter.
- The effects of temperature, osmolality and hydrogen ion concentration on the activation and acrosome reaction of golden hamster spermatozoa.
- Osmotic swelling of mammalian spermatozoa.
- The importance of serum albumin and metabolic intermediates for capacitation of spermatozoa and fertilization of mouse eggs in vitro.
- Fertilization of hamster ova by human spermatozoa in relation to other semen parameters.
- Inhibition of human sperm capacitation by a high-molecular-weight factor from human seminal plasma.
- Relationship between semen quality and human sperm penetration of zona-free hamster ova.
- Penetration of human spermatozoa into the human zona pellucida and the zona-free hamster egg: a study of fertile donors and infertile patients.
- Supravital staining of human spermatozoa.
- Analysis of human spermatozoal fertilizing ability using zona-free ova.
- Volume of stressed bull spermatozoa and protoplasmic droplets, and the relationship of cell size to motilityd fertility.
Cited by
- Hypo-osmotic swelling test: analysis of prevasectomy ejaculates.
- Improved preservation of rat epididymal sperm for high‐resolution low‐voltage scanning electron microscopy (HR‐LVSEM)
- Hypo‐osmotic swelling test: A reliable screening assay for routine semen in specimen quality screening
- Pregnancy following intracytoplasmic sperm injection of immotile spermatozoa selected by the hypo‐osmotic swelling‐test: a case report
- Effect of abnormal hypo-osmotic swelling test on fertilization rate and pregnancy outcome in in vitro fertilization cycles.
- Evaluation of dog semen quality after slow (biological freezer) or rapid (nitrogen vapours) freezing.
- Characteristics of seminal plasma proteins and their correlation with canine semen analysis
- Prognostic value of various spermatological attributes as predictors of zona binding and zona penetration of buffalo (Bubalus bubalis) semen.
- Influence of recovery method and microbial contamination on the response to freezing-thawing in ibex (Capra pyrenaica) epididymal spermatozoa.
- Response of buffalo spermatozoa to low temperatures during cryopreservation.
- Assessment of sperm survival and functional membrane integrity of the six-banded armadillo (Euphractus sexcinctus).
- The hypo-osmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia.
- Role of testosterone and photoperiod on seasonal changes in horn growth and sperm variables in the Iberian ibex: a model for polygynous wild bovids.
- Cellular Cargo Delivery: Toward Assisted Fertilization by Sperm-Carrying Micromotors.
- Effect on post-cryopreserved semen characteristics of Holstein bulls of adding combinations of vitamin C and either catalase or reduced glutathione to Tris extender.
- Effect of egg yolk powder on freezability of Murrah buffalo (Bubalus bubalis) semen
- The hypo-osmotic swelling test for evaluation of sperm membrane integrity.
- Processing and selection of surgically-retrieved sperm for ICSI: a review
- Effect of sodium nitroprusside on sperm motility, viability, and lipid peroxidation.
- Leukocytospermia is associated with poor semen quality.
Related papers
- Experimental Study on Confined Swelling Characteristic of Redbed Mudstone in Chongqing
- Polymer particle erosion controlling drug release. II. Swelling investigations to clarify the release mechanism.
- Water swelling behaviors of silica-reinforced NBR composites in deionized water and salt solution
- Influence of Leukocyte on Semen Quality of Infertile Men
- Evaluation of rat sperm by flow cytometry: simultaneous analysis of sperm count and sperm viability.
- Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation
- Flow cytometric analysis for sperm viability and counts in rats treated with trimethylphosphate or pyridoxine.
- Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm.
- Relationship between The Addition of Glutathione to Sperm Freezing Medium and Cryosurvival Rate of Sperm Motility and Viability in Asthenozoospermic Patients
- Hypo-osmotic swelling can accurately assess the viability of nonmotile sperm.