EBV transformation and cell culturing destabilizes DNA methylation in human lymphoblastoid cell lines.
Explore this paper's citation graph
Summary
The results suggest that lymphoblastoid cell lines should be used with caution for the identification of disease-associated DNA methylation changes or for discovery of new imprinted genes, as the methylation patterns seen in these cell lines may not always be representative ofDNA methylation present in the original B-lymphocytes of the patient.
- Type
- article
- Published
- 2010-02-01
- Cited by
- 122
- References
- 37
- Access
- Open access
- OpenAlex
- https://openalex.org/W1998914928
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:20994986
Keywords
DNA methylation, Biology, Epigenetics, Methylation, CpG site
References
- Genomic imprinting and imprinting defects in humans.
- Transcription factor LKLF is sufficient to program T cell quiescence via a c-Myc–dependent pathway
- Imprinting in human disease with special reference to transient neonatal diabetes and Beckwith-Wiedemann syndrome.
- Genomic structure and promoter characterization of an imprinted tumor suppressor gene ARHI.
- Evidence for an instructive mechanism of de novo methylation in cancer cells
- In Vitro Analysis of Integrated Global High-Resolution DNA Methylation Profiling with Genomic Imbalance and Gene Expression in Osteosarcoma
- Imprinted segments in the human genome: different DNA methylation patterns in the Prader-Willi/Angelman syndrome region as determined by the genomic sequencing method.
- Distribution, silencing potential and evolutionary impact of promoter DNA methylation in the human genome
- Extreme Clonality in Lymphoblastoid Cell Lines with Implications for Allele Specific Expression Analyses
- Mechanisms regulating imprinted genes in clusters.
- DNA methylation decreases in aging but not in immortal cells.
- MicroRNA expression changes during human leukemic HL-60 cell differentiation induced by 4-hydroxynonenal, a product of lipid peroxidation.
- Allele-specific methylation of a functional CTCF binding site upstream of MEG3 in the human imprinted domain of 14q32
- The neuronatin gene resides in a "micro-imprinted" domain on human chromosome 20q11.2.
- Widespread Monoallelic Expression on Human Autosomes
- A routine method for the establishment of permanent growing lymphoblastoid cell lines
- Altered gene expression and methylation of the human chromosome 11 imprinted region in small for gestational age (SGA) placentae.
- Identification of a large novel imprinted gene cluster on mouse proximal chromosome 6.
- Methylation dynamics of imprinted genes in mouse germ cells.
- IRAK-4/MyD88-dependent pathways are essential for the removal of developing autoreactive B cells in humans
Cited by
- Epstein-barr virus transformed DNA as a source of false positive findings in methylation studies of psychiatric conditions.
- Tyrosinemia type I as a model for studying epigenetic events in the aetiology of metabolic disease associated hepatocarcinoma
- CELLULAR PATHWAYS LEADING TO PATTERNS OF LYTIC EPSTEIN-BARR VIRUS REACTIVATION IN IMMORTALIZED B CELL LINES
- Monoallelic gene expression in human neural stem cells
- The presence of Epstein-Barr virus significantly impacts the transcriptional profile in immunodeficiency-associated Burkitt lymphoma
- Destabilization of the human epigenome: consequences of foreign DNA insertions.
- Epigenetic Epidemiology: Promises for Public Health Research
- Adult porcine genome-wide DNA methylation patterns support pigs as a biomedical model
- Genome-wide analysis of chicken miRNAs and DNA methylation and their roles in Marek's disease resistance and susceptibility
- Network signatures of cellular immortalization in human lymphoblastoid cell lines.
- Variation in Array Size, Monomer Composition and Expression of the Macrosatellite DXZ4
- Identification of Disease-Associated DNA Methylation in B Cells from Crohn’s Disease and Ulcerative Colitis Patients
- Epigenetics, a key for unlocking complex CNS disorders? Therapeutic implications.
- WNT2 promoter methylation in human placenta is associated with low birthweight percentile in the neonate
- DNMT gene expression and methylome in Marek’s disease resistant and susceptible chickens prior to and following infection by MDV
- Histone Deacetylases Suppress CGG Repeat–Induced Neurodegeneration Via Transcriptional Silencing in Models of Fragile X Tremor Ataxia Syndrome
- Comprehensive DNA methylation analysis of human peripheral blood leukocytes and lymphoblastoid cell lines
- Promoter Methylation in Head and Neck Squamous Cell Carcinoma Cell Lines Is Significantly Different than Methylation in Primary Tumors and Xenografts
- Assessment of methylation level prediction accuracy in methyl-DNA immunoprecipitation and sodium bisulfite based microarray platforms
- Immortalization of T-Cells Is Accompanied by Gradual Changes in CpG Methylation Resulting in a Profile Resembling a Subset of T-Cell Leukemias
Related papers
- Comparison of methyl-DNA immunoprecipitation (MeDIP) and methyl-CpG binding domain (MBD) protein capture for genome-wide DNA methylation analysis reveal CpG sequence coverage bias
- Genome-wide DNA methylation profiling using the methylation-dependent restriction enzyme LpnPI
- Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization
- MIRA-SNuPE, a quantitative, multiplex method for measuring allele-specific DNA methylation
- The roles of DNA methylation on pH dependent i-motif (iM) formation in rice
- P2‐011: DNA methylation in brain tissue and Alzheimer's disease
- Epigenetics: the DNA methylation profile of tissue-dependent and differentially methylated regions in cells.
- Abstract LB-133: Genome-wide differences in DNA methylation between tumor and normal adjacent tissue mapped by high-throughput sequencing of methyl-CPG binding domain based affinity-purified genomic fragments
- MIRA-seq for DNA methylation analysis of CpG islands