Real-time DNA sequencing using detection of pyrophosphate release.
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Summary
An approach for real-time DNA sequencing without the need for electrophoresis has been developed that relies on the detection of DNA polymerase activity by an enzymatic luminometric inorganic pyrophosphate (PPi) detection assay (ELIDA) and the possibility for parallel processing of many samples in an automated manner is discussed.
- Type
- article
- Published
- 1996-11-01
- Cited by
- 1,239
- References
- 0
- OpenAlex
- https://openalex.org/W1994057432
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:21096907
Keywords
Pyrophosphate, DNA polymerase, Chemistry, Molecular biology, Polymerase
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