The role of Gln61 and Glu63 of Ras GTPases in their activation by NF1 and Ras GAP.
Explore this paper's citation graph
Summary
The NF1C is demonstrated to be a highly specific for Ras and does not activate either Rap1 or Rho GTPases, and it is shown that Gln at position 61 of the GTPase primarily determines that NF1 C as well as GAP1C activates Ras GTPased, but not Rap1 GTPasing.
- Type
- article
- Published
- 1992-12-01
- Cited by
- 15
- References
- 43
- Access
- Open access
- OpenAlex
- https://openalex.org/W1989693623
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:30551453
Keywords
GTPase, Guanine nucleotide exchange factor, Cell biology, Biology, GTP'
References
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
- The residues of Ras and Rap proteins that determine their GAP specificities.
- The purification of a Rap1 GTPase-activating protein from bovine brain cytosol.
- Purification of GTPase-activating protein specific for the rho gene products.
- Asparagine 26, glutamic acid 31, valine 45, and tyrosine 64 of Ras proteins are required for their oncogenicity.
- Purification and characterization from bovine brain cytosol of two GTPase-activating proteins specific for smg p21, a GTP-binding protein having the same effector domain as c-ras p21s.
- Rap1A antagonizes the ability of Ras and Ras-Gap to inhibit muscarinic K+ channels.
- Rsr1 and Rap1 GTPases are activated by the same GTPase-activating protein and require threonine 65 for their activation.
- The cytoplasmic protein GAP is implicated as the target for regulation by the ras gene product
- ras p21 and GAP inhibit coupling of muscarinic receptors to atrial K+ channels.
- Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1.
- Purification of a plasma membrane-associated GTPase-activating protein specific for rap1/Krev-1 from HL60 cells.
- A ras-related gene with transformation suppressor activity.
- Xenopus oocyte germinal-vesicle breakdown induced by [Val12]Ras is inhibited by a cytosol-localized Ras mutant.
- The GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21.
- GAP domains responsible for ras p21-dependent inhibition of muscarinic atrial K+ channel currents.
- Analysis of the transforming potential of the human H-ras gene by random mutagenesis.
- Molecular cloning of a GTPase activating protein specific for the Krev-1 protein p21rap1.
- cDNA cloning of the type 1 neurofibromatosis gene: complete sequence of the NF1 gene product.
- Purification of ras GTPase activating protein from bovine brain.
Cited by
- Mutational analysis of KRAS and NRAS in metastatic colorectal cancer
- Site-Directed Mutagenesis of Thermus thermophilus Elongation Factor Tu
- INVESTIGATION OF THE ROLE OF RASGAP IN PROMOTING NEURONAL SURVIVAL IN DROSOPHILA
- Decreased GTPase activity of K- ras mutants deriving from human functional adrenocortical tumours
- Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression
- GTP-binding proteins. Structures, interactions and relationships.
- Site-directed mutagenesis of Thermus thermophilus elongation factor Tu. Replacement of His85, Asp81 and Arg300.
- Ras/Rap effector specificity determined by charge reversal
- A New Function of p120-GTPase-activating Protein
- Human OLA1 Defines an ATPase Subfamily in the Obg Family of GTP-binding Proteins*
- Overexpression and functional analysis of a mitogen-inducible nuclear GTPase activating protein, Spa-1.
- Myopathy caused by HRAS germline mutations: implications for disturbed myogenic differentiation in the presence of constitutive HRas activation
- Requirement of Activated Cdc42-Associated Kinase for Survival of v-Ras-Transformed Mammalian Cells
- Mammals II: Downstream of RAS and Actin-Cytoskeleton
Related papers
- Ras and Rho GTPases: a family reunion.
- Targeting the Small GTPase Superfamily through Their Regulatory Proteins
- Small GTPases of the Ras and Rho Families Switch on/off Signaling Pathways in Neurodegenerative Diseases
- Phosphatidic acid signaling regulation of Ras superfamily of small guanosine triphosphatases
- Keyword Index Volume 16, 2004
- Nitric oxide activates Rap1 and Ral in a Ras-independent manner.
- Divergent Mechanisms Activating RAS and Small GTPases Through Post-translational Modification
- Neutron Crystal Structure of RAS GTPase Puts in Question the Protonation State of the GTP γ-Phosphate*
- Overexpression and functional analysis of a mitogen-inducible nuclear GTPase activating protein, Spa-1.