Possible roles for a non-modular, thermostable and proteinase-resistant cellulase from the mesophilic aerobic soil bacterium Cellvibrio mixtus
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Summary
Analysis of the biochemical properties of CelA revealed that the enzyme hydrolyses a range of soluble cellulosic substrates, but was inactive against Avicel, xylan or any other hemicellulose, and suggests that CelA is a single-domain endoglucanase with no distinct non-catalytic cellulose-binding domain.
- Type
- article
- Published
- 1997-10-01
- Cited by
- 27
- References
- 34
- OpenAlex
- https://openalex.org/W1986834017
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:30421685
Keywords
Cellulase, Biochemistry, Cellulose, Bacteria, Hemicellulose
References
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- Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei
- DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca
- DNA sequences and expression in Streptomyces lividans of an exoglucanase gene and an endoglucanase gene from Thermomonospora fusca
- Cloning of the Thermomonospora fusca Endoglucanase E2 Gene in Streptomyces lividans: Affinity Purification and Functional Domains of the Cloned Gene Product
- New families in the classification of glycosyl hydrolases based on amino acid sequence similarities.
- Novel cellulose-binding domains, NodB homologues and conserved modular architecture in xylanases from the aerobic soil bacteria Pseudomonas fluorescens subsp. cellulosa and Cellvibrio mixtus.
- The non-catalytic cellulose-binding domain of a novel cellulase from Pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of Avicel.
- Reduction of intestinal viscosity through manipulation of dietary rye and pentosanase concentration is effected through changes in the carbohydrate composition of the intestinal aqueous phase and results in improved growth rate and food conversion efficiency of broiler chicks.
- ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE.
- Non–Hydrolytic Disruption of Cellulose Fibres by the Binding Domain of a Bacterial Cellulase
- The nature of the cellulose‐binding domain effects the activities of a bacterial endoglucanase on different forms of cellulose
- Transplanting Two Unique β-Glucanase Catalytic Activities Into One Multienzyme, Which Forms Glucose
- Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
- A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
- Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a β‐1,4‐exoceilobiohydrolase analogous to Trichoderma reesei CBH II
- Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
- Evidence for a general role for high‐affinity non‐catalytic cellulose binding domains in microbial plant ceil wall hydroiases
- Pseudomonas fluorescens subsp. cellulosa: an alternative model for bacterial cellulase.
- Molecular cloning of multiple xylanase genes from Pseudomonas fluorescens subsp. cellulosa.
Cited by
- Rational use of dietary enzymes and lipids to improve broiler performance and meat quality
- Screening for cellulase-encoding clones in metagenomic libraries.
- Effect of cellulase/glucanase/xylanase (Roxazyme G2) enzymes combination on nutrients utilisation of vegetable meal (Amaranthus cruentus) fed as sole dietary protein source in rat assay
- A novel Cellvibrio mixtus family 10 xylanase that is both intracellular and expressed under non-inducing conditions.
- Characterization of a metagenome-derived halotolerant cellulase.
- Expression of the Genes celA and xylA isolated from a fragment of metagenomic DNA in Escherichia coli
- Levels of endogenous β-glucanase activity in barley affect the efficacy of exogenous enzymes used to supplement barley-based diets for poultry.
- Bioconversion and enzymes fortification of palm kernel meal as protein supplement in broiler rations.
- Cloning and functional characterization of endo-β-1,4-glucanase gene from metagenomic library of vermicompost
- Molecular cloning, purification, and characterization of a novel, acidic, pH-stable endoglucanase from Martelella mediterranea
- Identification of tandemly repeated type VI cellulose-binding domains in an endoglucanase from the aerobic soil bacterium Cellvibrio mixtus
- Proteins for breaking barriers in lignocellulosic bioethanol production.
- Isolation and identification of cellulolytic bacteria involved in the degradation of natural cellulosic fibres.
- Nutritional evaluation of Amaranthus cruentus leaf meal based broiler diets supplemented with cellulase/glucanase/xylanase enzymes.
- A family 6 carbohydrate-binding module potentiates the efficiency of a recombinant xylanase used to supplement cereal-based diets for poultry
- The Pseudomonas cellulosa glycoside hydrolase family 51 arabinofuranosidase exhibits wide substrate specificity.
- Use of Cellulases and Xylanases to Supplement Diets Containing Alfalfa for Broiler Chicks: Effects on Bird Performance and Skin Color
- Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene
- Use of β-Glucanases and β-1,4-Xylanases to Supplement Diets Containing Alfalfa and Rye for Laying Hens: Effects on Bird Performance and Egg Quality
- Xylanase Inhibitors Affect the Action of Exogenous Enzymes Used to Supplement Triticum durum-Based Diets for Broiler Chicks
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