Sequencing of first-strand cDNA library reveals full-length transcriptomes
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Summary
A novel method for ssRNA-seq that enables the simultaneous profiling of gene expression, TSSs and polyadenylation sites at near-base resolution with a single library is presented.
- Type
- article
- Published
- 2015-01-21
- Cited by
- 24
- References
- 58
- Access
- Open access
- OpenAlex
- https://openalex.org/W1984874059
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:205334261
Keywords
Polyadenylation, Transcriptome, Complementary DNA, cDNA library, Biology
References
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- The nuclear RNase III Drosha initiates microRNA processing
- Solid-phase reversible immobilization for the isolation of PCR products.
- Alternative cleavage and polyadenylation: extent, regulation and function
- Precise Maps of RNA Polymerase Reveal How Promoters Direct Initiation and Pausing
- RNA Exosome Depletion Reveals Transcription Upstream of Active Human Promoters
- Nascent RNA Sequencing Reveals Widespread Pausing and Divergent Initiation at Human Promoters
- Noncanonical transcript forms in yeast and their regulation during environmental stress.
- Smart-seq2 for sensitive full-length transcriptome profiling in single cells
- Digital transcriptome profiling using selective hexamer priming for cDNA synthesis
- Condensin controls recruitment of RNA polymerase II to achieve nematode X-chromosome dosage compensation
- A Multiplex RNA-seq Strategy to Profile Poly(A+) RNA: Application to Analysis of Transcription Response and 3′ End Formation
- The Antisense Transcriptomes of Human Cells
- Divergent transcription from active promoters
- High-efficiency cloning of full-length cDNA
Cited by
- Altered Gene-Regulatory Function of KDM5C by a Novel Mutation Associated With Autism and Intellectual Disability
- Systematic Identification of Factors for Provirus Silencing in Embryonic Stem Cells
- A mouse model of X-linked intellectual disability associated with impaired removal of histone methylation
- Transcriptome analysis revealed impaired cAMP responsiveness in PHF21A-deficient human cells
- LSD1/KDM1A Maintains Genome-wide Homeostasis of Transcriptional Enhancers
- Capturing the ‘ome’: the expanding molecular toolbox for RNA and DNA library construction
- SL-quant: a fast and flexible pipeline to quantify spliced leader trans-splicing events from RNA-seq data
- Screening a Spliced Leader-Based Symbiodinium microadriaticum cDNA Library Using the Yeast-Two Hybrid System Reveals a Hemerythrin-Like Protein as a Putative SmicRACK1 Ligand
- An ultra low-input method for global RNA structure probing uncovers Regnase-1-mediated regulation in macrophages
- PTT-quant: a new method for direct identification and absolute quantification of premature transcription termination events, following the example of bacterial riboswitches
- Epitranscriptomic mapping of RNA modifications at single-nucleotide resolution using rhodamine sequencing (Rho-seq)
- Alcohol-specific transcriptional dynamics of memory reconsolidation
- Alcohol-specific transcriptional dynamics of memory reconsolidation and relapse
- Tn5 tagments and transposes oligos to single-stranded DNA for strand-specific RNA sequencing
- Transcriptome profile analysis of Indian mustard (Brassica juncea L.) during seed germination reveals the drought stress-induced genes associated with energy, hormone, and phenylpropanoid pathways.
- Type I/type III IFN and related factors regulate JEV infection and BBB endothelial integrity
- Elucidating the regulatory role of long non-coding RNAs in drought stress response during seed germination in leaf mustard
- Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complex
- Biomolecules Interacting with Long Noncoding RNAs
- Insights on the impact of arbuscular mycorrhizal symbiosis on Avena sativa drought tolerance at the early flowering stage.
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