A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry.
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Summary
A flow cytometric method for measuring the percentage of apoptotic nuclei after propidium iodide staining in hypotonic buffer is developed and shown an excellent correlation with the results obtained with both electrophoretic and colorimetric methods.
- Type
- article
- Published
- 1991-06-03
- Cited by
- 4,812
- References
- 15
- OpenAlex
- https://openalex.org/W1979129905
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:5796744
Keywords
Propidium iodide, Apoptosis, Molecular biology, Thymocyte, DNA fragmentation
References
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- Practical Flow Cytometry
- Calcium‐activated DNA fragmentation kills immature thymocytes
- Activation of a suicide process of thymocytes through DNA fragmentation by calcium ionophores and phorbol esters.
- Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death.
- Cell death: the significance of apoptosis.
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
- Monoclonal antibody-mediated tumor regression by induction of apoptosis.
- Protein kinase-C involvement in thymocyte apoptosis induced by hydrocortisone.
- Antibodies to CD3/T-cell receptor complex induce death by apoptosis in immature T cells in thymic cultures
- Rapid hypotonic method for flow cytofluorometry of monolayer cell cultures. Some pitfalls in staining and data analysis.
- Cyclosporin A inhibits activation-induced cell death in T-cell hybridomas and thymocytes
- Simultaneous cell surface phenotype and cell cycle analysis of lymphocytes by flow cytometry.
- Haemopoietic colony stimulating factors promote cell survival by suppressing apoptosis
- Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis
Cited by
- Promyelocytic leukemia zinc finger protein (PLZF) enhances glucocorticoid-induced apoptosis in leukemic cell line NALM6.
- Induction of apoptotic cell death in mouse lymphoma and human leukemia cell lines by a calcium‐binding protein complex, calprotectin, derived from inflammatory peritoneal exudate cells
- Taxol cytotoxicity on human leukemia cell lines is a function of their susceptibility to programmed cell death
- Interactions between B lymphocytes and NK cells
- Catecholamines are synthesized by mouse lymphocytes and regulate function of these cells by induction of apoptosis
- Study of apoptosis in human lymphocytes by toxic substances implicated in toxic oil syndrome.
- Combined immunodeficiency phenotype associated with inappropriate spontaneous and activation‐induced apoptosis
- Antiphosphatidylserine antibodies in human immunodeficiency virus-1 patients with evidence of T-cell apoptosis and mediate antibody-dependent cellular cytotoxicity.
- Cloning and expression of a murine histone deacetylase 3 (mHdac3) cDNA and mapping to a region of conserved synteny between murine chromosome 18 and human chromosome 5.
- Identification of a Novel Tumor Necrosis Factor-α-inducible Gene, SCC-S2, Containing the Consensus Sequence of a Death Effector Domain of Fas-associated Death Domain-like Interleukin- 1β-converting Enzyme-inhibitory Protein*
- The fas antigen is involved in thymic T-cell development as a costimulatory molecule, but not in the deletion of neglected thymocytes.
- Induction of apoptosis by a novel intestinal metabolite of ginseng saponin via cytochrome c-mediated activation of caspase-3 protease.
- TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma Is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria1
- CD44 supports T cell proliferation and apoptosis by apposition of protein kinases
- Antiapoptotic effects of IL-15 on pulmonary Tc1 cells of patients with human immunodeficiency virus infection.
- Pharmacological separation of the expression of tissue transglutaminase and apoptosis after chemotherapeutic treatment of HepG2 cells.
- Unsaturated Fatty Acid-mediated Decreases in Sterol Regulatory Element-mediated Gene Transcription Are Linked to Cellular Sphingolipid Metabolism*
- Radiation sensitivity depends on OGG1 activity status in human leukemia cell lines.
- Factor(s) released from irradiated B-CLL cells induce apoptosis in leukemic lymphocytes.
- Role for Bcl-6 in the generation and maintenance of memory CD8+ T cells
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