A large-scale method to measure absolute protein phosphorylation stoichiometries
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Summary
An integrated method that relies on phosphatase treatment and stable-isotope labeling to determine absolute stoichiometries of protein phosphorylation on a large scale, which requires the measurement of only a single ratio relating phosph atase-treated and mock-treated samples.
- Type
- article
- Published
- 2011-07-03
- Cited by
- 287
- References
- 44
- Access
- Open access
- OpenAlex
- https://openalex.org/W1977637361
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:8904854
Keywords
Stoichiometry, Phosphorylation, Phosphatase, Protein phosphatase 1, Phase (matter)
References
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- High-performance liquid chromatography/electrospray ionization ion-trap mass spectrometry for analysis of oligosaccharides derivatized by reductive amination and N,N-dimethylation.
- Analytical strategies for phosphoproteomics
- Principles and Applications
- Identification and characterization of cellular targets for tyrosine protein kinases.
- Length-dependent prediction of protein intrinsic disorder
- Studies of ligand-induced site-specific phosphorylation of epidermal growth factor receptor
- Systematic identification of mitotic phosphoproteins.
- Assay Development for the Determination of Phosphorylation Stoichiometry using MRM methods with and without Phosphatase Treatment: Application to Breast Cancer Signaling Pathways
- The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
- An isotope labeling strategy for quantifying the degree of phosphorylation at multiple sites in proteins
- Measurement of protein phosphorylation stoichiometry by selected reaction monitoring mass spectrometry.
- An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database
- Stoichiometric Quantification of Akt Phosphorylation Using LC-MS/MS
- Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes.
- Correct Interpretation of Comprehensive Phosphorylation Dynamics Requires Normalization by Protein Expression Changes*
- Structural framework for the protein kinase family.
- Phosphopeptide fragmentation and analysis by mass spectrometry.
- Quantification of Protein Phosphorylation by Liquid Chromatography−Mass Spectrometry
- Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS
Cited by
- Enzymatic Tagging of Glycoproteins on the Cell Surface for Their Global and Site-Specific Analysis with Mass Spectrometry
- Mass spectrometric tools for systematic analysis of protein phosphorylation.
- QUANTITATIVE CHARACTERIZATION OF PROTEINS AND POST-TRANSLATIONAL MODIFICATIONS IN COMPLEX PROTEOMES USING HIGH-RESOLUTION MASS SPECTROMETRY-BASED PROTEOMICS
- Qualitative and quantitative analysis of post-translational modifications of chromatin in the mouse brain and sperm
- A robust algorithm for segmenting fluorescence images and its application tosingle-molecule counting
- Advancements of Mass Spectrometry in Biomedical Research
- Uncovering dynamic phosphorylation signaling using mass spectrometry
- Quantifying Ubiquitin Signaling
- The Promise of Proteomics for the Study of ADP-ribosylation
- Multiplex staining of 2-DE gels for an initial phosphoproteome analysis of germinating seeds and early grown seedlings from a non-orthodox specie: Quercus ilex L. subsp. ballota [Desf.] Samp.
- Global Analysis of the Serine, Threonine and Tyrosine Phosphorylation Networks in the Model Bacterium Bacillus subtilis
- Protein Phosphorylation: A Major Switch Mechanism for Metabolic Regulation.
- Mass spectrometry‐driven phosphoproteomics: patterning the systems biology mosaic
- Recent findings and technological advances in phosphoproteomics for cells and tissues
- Experimental and Computational Tools for Analysis of Signaling Networks in Primary Cells
- Spectrométrie de masse des modifications induites ou post-traductionnelles de protéines : méthodologie et application à des protéines d’intérêt thérapeutique
- Multiscale analysis of the murine intestine for modeling human diseases
- Recent advances and challenges in plant phosphoproteomics
- Accurate determination of Peptide Phosphorylation Stoichiometry Via Automated Diagonal Capillary Electrophoresis Coupled with Mass Spectrometry – Proof of Principle
- Computational phosphoproteomics: From identification to localization
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