Droplet-based microfluidic platforms for the encapsulation and screening of Mammalian cells and multicellular organisms.
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Summary
Droplet-based microfluidic platforms in which cells are grown in aqueous microcompartments separated by an inert perfluorocarbon carrier oil are described, which should open the way for high-throughput, cell-based screening that can use >1000-fold smaller assay volumes and has approximately 500x higher throughput than conventional microtiter plate assays.
- Type
- article
- Published
- 2008-05-19
- Cited by
- 710
- References
- 45
- Access
- Open access
- OpenAlex
- https://openalex.org/W1975581000
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:16072206
Keywords
Microfluidics, Microtiter plate, Multicellular organism, High-throughput screening, Miniaturization
References
- Quantitative detection of protein expression in single cells using droplet microfluidics.
- An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids.
- Utility of lab‐on‐a‐chip technology for high‐throughput nucleic acid and protein analysis
- Perfluorochemicals: their applications and benefits to cell culture.
- Optical barcoding of colloidal suspensions: applications in genomics, proteomics and drug discovery.
- Dynamic pattern formation in a vesicle-generating microfluidic device.
- Higher Viscous Solution Induces Smaller Droplets for Cell‐Enclosing Capsules in a Co‐flowing Stream
- Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome
- Dielectrophoretic manipulation of drops for high-speed microfluidic sorting devices
- A novel lentivirus vector derived from apathogenic simian immunodeficiency virus.
- Intracellular detection assays for high-throughput screening.
- Formation of dispersions using “flow focusing” in microchannels
- Water-in-Carbon Dioxide Microemulsions: An Environment for Hydrophiles Including Proteins
- Microfluidics: Fluid physics at the nanoliter scale
- Generation of larger numbers of separated microbial populations by cultivation in segmented-flow microdevices.
- Multifunctional Encoded Particles for High-Throughput Biomolecule Analysis
- Digital microfluidics with in-line sample purification for proteomics analyses with MALDI-MS.
- Cellular platforms for HTS: three case studies.
- Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets.
- Production of arrays of chemically distinct nanolitre plugs via repeated splitting in microfluidic devices.
Cited by
- Surfactants in droplet-based microfluidics.
- Microfluidic Approaches to Synchrotron Radiation-Based Fourier Transform Infrared (SR-FTIR) Spectral Microscopy of Living Biosystems
- Droplet‐based microfluidics systems in biomedical applications
- Integrated manipulation, detection and counting of cells in biofluids
- Massively parallel analysis of cells and nucleic acids
- Droplet microfluidics for single-cell analysis.
- Innovations in Imaging System Design: Gigapixel, Chip-Scale and MultiFunctional Microscopy
- Microwell devices for single-cell analyses
- Microdroplet-enabled co-cultivation and characterization of microbial communities
- Droplets as reaction compartments for protein nanotechnology.
- Deterministic Lateral Displacement for Characterization of Bacterial Secretion
- Next generation digital microfluidic technology: Electrophoresis of charged droplets
- Dynamic control and quantification of bacterial population dynamics in droplets
- Monitoring biochemical reactions in stationary droplets
- Fluorescence picoseconde de complexes bio-moléculaires hors équilibre dans un écoulement microfluidique
- Microfluidic Diagnostics
- In vivo and in vitro directed evolution of enzymes using droplet-based microfluidics
- Stabilisers for water-in-fluorinated-oil dispersions: Key properties for microfluidic applications
- Microfluidic engineering of artificial stem cell niches
- Ultrasound-triggered drug delivery using acoustic droplet vaporization
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