Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing

Keywords

Dimer, Chemistry, Isotopic labeling, RNA splicing, Escherichia coli

References

• Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteinsOtto Laske — 2010
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• Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studiesSara Züger, Hideo Iwaï — 2005
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• Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitroMikko Muona, A. Sesilja Aranko, Vytas Raulinaitis — 2010
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• Structure and dynamics of a CheY-binding domain of the chemotaxis kinase CheA determined by nuclear magnetic resonance spectroscopy.Megan M. McEvoy, D.R. Muhandiram, Lewis E. Kay — 1996
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Cited by

• Tandem SUMO fusion vectors for improving soluble protein expression and purification.Fernando Guerrero, Annika Ciragan, Hideo Iwaï — 2015
• Intermolecular protein splicing and its use in biotechnological applicationsA. Sesilja Aranko — 2014
• Plasticity of the β-barrel assembly machinery investigated by NMRTessa Sinnige — 2014
• NMR approaches for structural analysis of multidomain proteins and complexes in solution.Christoph Göbl, Tobias Madl, Bernd Simon — 2014
• Structural basis for protein trans‐splicing by a bacterial intein‐like domain – protein ligation without nucleophilic side chainsA. Sesilja Aranko, Jesper S. Oeemig, Hideo Iwaï — 2013
• Structure-based engineering and comparison of novel split inteins for protein ligation†A. Sesilja Aranko, Jesper S. Oeemig, Dongwen Zhou — 2014
• SPLICEFINDER – A Fast and Easy Screening Method for Active Protein Trans-Splicing PositionsJ. L. Zettler, Simone Eppmann, Alena Busche — 2013
• Solution structure of the two RNA recognition motifs of hnRNP A1 using segmental isotope labeling: how the relative orientation between RRMs influences the nucleic acid binding topologyPierre Barraud, Frédéric H.‐T. Allain — 2012
• Segmental isotope‐labeling of the intrinsically disordered protein PQBP1Yuko Nabeshima, Mineyuki Mizuguchi, Asagi Kajiyama — 2014
• Structural Studies on InteinsJesper S Øemig — 2013
• Amino Acid‐Selective Segmental Isotope Labeling of Multidomain Proteins for Structural BiologyErich Michel, Lenka Skříšovská, Kurt Wüthrich — 2013
• Semi-Synthesis of Labeled Proteins for Spectroscopic ApplicationsLucía De Rosa, Anna Russomanno, Alessandra Romanelli — 2013
• Split-inteins and their bioapplicationsYifeng Li — 2015
• Dual modification of biomolecules.Antoine Maruani, Daniel A. Richards, Vijay Chudasama — 2016
• Segmental Isotopic Labeling of Proteins for NMR Study Using Intein Technology.Dongsheng Liu, David Cowburn — 2017
• Salt-inducible Protein Splicing in cis and trans by Inteins from Extremely Halophilic Archaea as a Novel Protein-Engineering Tool.Annika Ciragan, A. Sesilja Aranko, Igor Tascón — 2016
• Segmental Isotope Labeling of Insoluble Proteins for Solid-State NMR by Protein Trans-Splicing.Tobias Schubeis, Madhu Nagaraj, Christiane Ritter — 2017
• Structural studies of potential new-generation antibiotic targets and the use of one of them, TonB as a model protein in protein engineeringAnnika Ciragan — 2017
• Segmental isotopic labeling of a single‐domain globular protein without any refolding step by an asparaginyl endopeptidaseKornelia M. Mikula, Igor Tascón, Jenni J. Tommila — 2017
• Dynamic domain arrangement of CheA-CheY complex regulates bacterial thermotaxis, as revealed by NMRYuichi Minato, Takumi Ueda, Asako Machiyama — 2017

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