Transformation of intact yeast cells treated with alkali cations.
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Summary
The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.
- Type
- article
- Published
- 1984-02-01
- Cited by
- 4,220
- References
- 15
- Access
- Open access
- OpenAlex
- https://openalex.org/W1973782344
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:463309
Keywords
Plasmid, Transformation (genetics), Protoplast, Saccharomyces cerevisiae, Transformation efficiency
References
- Fermentative Formation of CDP-Choline by Intact Cells of a Yeast, Saccharomyces carlsbergensis (IFO 0641) Treated with a Detergent, Triton X-100
- Fusion of yeast spheroplasts
- Analysis of Endonuclease R·EcoRI Fragments of DNA from Lambdoid Bacteriophages and Other Viruses by Agarose-Gel Electrophoresis
- Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid
- Transformation of yeast.
- High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
- High-frequency transformation of the fission yeast Schizosaccharomyces pombe
- Transfer of mitochondria by protoplast fusion in Saccharomyces cerevisiae
- Analysis of chromosomal integration and deletions of yeast plasmids.
- Calcium-dependent bacteriophage DNA infection.
- Uptake of Single-Stranded Bacteriophage DNA by Isolated Tobacco Protoplasts
- Biofunctional Change in Yeast Cell Surface on Treatment with Triton X-100
- Nucleic acid transport driven by ion gradient across cell membrane
- Biofunctional Change in Yeast Cell Surface on Treatment with Triton X-100
Cited by
- Dihydrofolate reductase mutations in Plasmodium vivax from Indonesia and therapeutic response to sulfadoxine plus pyrimethamine.
- Connections between RNA splicing and DNA intron mobility in yeast mitochondria: RNA maturase and DNA endonuclease switching experiments
- A replication map of a 61-kb circular derivative of Saccharomyces cerevisiae chromosome III.
- Identification of a human cDNA encoding a protein that is structurally and functionally related to the yeast adenylyl cyclase-associated CAP proteins
- Role of GCR2 in transcriptional activation of yeast glycolytic genes
- A gene encoding a protein with seven zinc finger domains acts on the sexual differentiation pathways of Schizosaccharomyces pombe.
- Dissection of a carboxy-terminal region of the yeast regulatory protein RAP1 with effects on both transcriptional activation and silencing
- Yeast telomere repeat sequence (TRS) improves circular plasmid segregation, and TRS plasmid segregation involves the RAP1 gene product
- N‐linked glycosylation of proteinase B precursors of the yeast Saccharomyces cerevisiae is not require for proper targeting or processing of the enzyme
- The NSR1 gene encodes a protein that specifically binds nuclear localization sequences and has two RNA recognition motifs
- Characterization of double-strand break-induced recombination: homology requirements and single-stranded DNA formation
- RSR1, a ras-like gene homologous to Krev-1 (smg21A/rap1A): role in the development of cell polarity and interactions with the Ras pathway in Saccharomyces cerevisiae
- Electroporation‐stimulated recombination in yeast
- RPD1 (SIN3/UME4) is required for maximal activation and repression of diverse yeast genes
- Preparation of clone libraries in yeast artificial-chromosome vectors.
- CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae
- Drosophila scaffold-attached regions bind nuclear scaffolds and can function as ARS elements in both budding and fission yeasts
- Loss of Ras activity in Saccharomyces cerevisiae is suppressed by disruptions of a new kinase gene, YAKI, whose product may act downstream of the cAMP-dependent protein kinase.
- Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm.
- Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes
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