Transformation of intact yeast cells treated with alkali cations.

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Summary

The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.

Type
article
Published
1984-02-01
Cited by
4,220
References
15
Access
Open access

Keywords

Plasmid, Transformation (genetics), Protoplast, Saccharomyces cerevisiae, Transformation efficiency

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