Fluorescence detection in automated DNA sequence analysis
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Summary
A method for the partial automation of DNA sequence analysis by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymaticDNA sequence analysis.
- Type
- article
- Published
- 1986-06-12
- Cited by
- 1,666
- References
- 7
- OpenAlex
- https://openalex.org/W1969757078
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:27800972
Keywords
Fluorophore, Oligonucleotide, DNA, Primer (cosmetics), Fluorescence
References
- Sequencing end-labeled DNA with base-specific chemical cleavages.
- Oligonucleotide synthesis : a practical approach
- DNA sequence analysis by primed synthesis.
- Solid-phase methods for sequencing of nucleic acids I. Simultaneous sequencing of different oligodeoxyribonucleotides using a new, mechanically stable anion-exchange paper.
- The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis.
- Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin.
- DNA sequencing with chain-terminating inhibitors.
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- Imaging as a tool for improving length and accuracy of sequence analysis in automated fluorescence‐based DNA sequencing
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- Genomic Organization and Chromosomal Localization of the Gene Encoding Human P-selectin Glycoprotein Ligand (*)
- An infrared fluorescent dATP for labeling DNA.
- Base-calling of automated sequencer traces using phred. I. Accuracy assessment.
- Molecular genetic applications of streptavidin-coated manifold supports.
- Antibody reactivity to HIV-1 Vpu in HIV-1/AIDS patients on highly active antiretroviral therapy.
- Applications de la spectrométrie de masse type MALDI-TOF à la bactériologie et à la distinction de variants génétiques
- Phylogenetic Analysis of the Sigma 2 Protein Gene of Turkey Reoviruses
- Presentation, Patterns of Myocardial Damage, and Clinical Course of Viral Myocarditis
- An overview of recent developments in genomics and associated statistical methods
- A review of methods for detect human Papillomavirus infection
- Assembly algorithms for deep sequencing data: basics and pitfalls.
- Ray Wu as Fifth Business: Deconstructing collective memory in the history of DNA sequencing.
- Anomalous electrophoretic migration of short oligodeoxynucleotides labelled with 5′‐terminal Cy5 dyes
- Proteogenomics: Integrating Next-Generation Sequencing and Mass Spectrometry to Characterize Human Proteomic Variation
- Beyond the standard of care: the role of cytopathology in molecular testing of cancer.
- Advances in DNA Detection
- Ultra-thin-layer agarose gel electrophoresis. I. Effect of the gel concentration and temperature on the separation of DNA fragments.
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