NMR structure determination of a segmentally labeled glycoprotein using in vitro glycosylation.
Explore this paper's citation graph
Summary
A novel method of NMR structural determination that overcomes some of the problems of inhomogeneous and incomplete glycosylation, and extends the observable NOE distance limit of oligosaccharides significantly over 4 A, resulting in a high number of distance restraints per glycosidic linkage.
- Type
- article
- Published
- 2009-01-28
- Cited by
- 69
- References
- 59
- OpenAlex
- https://openalex.org/W1968701309
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:207134941
Keywords
Chemistry, Glycosylation, Glycoprotein, In vitro, Biophysics
References
- Characterisation by 1H-n.m.r. spectroscopy of oligosaccharides, derived from arabinoxylans of white endosperm of wheat, that contain the elements ----4)[alpha-L-Araf-(1----3)]-beta-D-Xylp-(1---- or ----4)[alpha- L-Araf-(1----2)][alpha-L-Araf-(1----3)]-beta-D-Xylp-(1----.
- Computational carbohydrate chemistry: what theoretical methods can tell us
- Protein NMR structure determination with automated NOE-identification in the NOESY spectra using the new software ATNOS
- Carbohydrate Structure Suite (CSS): analysis of carbohydrate 3D structures derived from the PDB
- Protein backbone angle restraints from searching a database for chemical shift and sequence homology
- Conformational preferences of chondroitin sulfate oligomers using partially oriented NMR spectroscopy of 13C-labeled acetyl groups.
- The high degree of internal flexibility observed for an oligomannose oligosaccharide does not alter the overall topology of the molecule.
- Gradient-Tailored Water Suppression for 1H-15N HSQC Experiments Optimized to Retain Full Sensitivity
- Chemical synthesis of a glycoprotein having an intact human complex-type sialyloligosaccharide under the Boc and Fmoc synthetic strategies.
- Effects of glycosylation on peptide conformation: a synergistic experimental and computational study.
- Statistical Basis for the Use of13CαChemical Shifts in Protein Structure Determination
- Structural basis of high-affinity glycan recognition by bacterial and fungal lectins.
- MUSIC in triple-resonance experiments: amino acid type-selective (1)H-(15)N correlations
- From peptide to protein: comparative analysis of the substrate specificity of N-linked glycosylation in C. jejuni.
- Correlating Backbone Amide and Side-Chain Resonances in Larger Proteins By Multiple Relayed Triple Resonance NMR
- Effects of the N-Linked Glycans on the 3D Structure of the Free α-Subunit of Human Chorionic Gonadotropin†
- Structure determination of a tetrasaccharide: transient nuclear Overhauser effects in the rotating frame
- MOLMOL: a program for display and analysis of macromolecular structures.
- Towards understanding the interaction between oligosaccharides and water molecules.
- Correlated C-C and C-O bond conformations in saccharide hydroxymethyl groups: parametrization and application of redundant 1H-1H, 13C-1H, and 13C-13C NMR J-couplings.
Cited by
- Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins
- Segmental labeling to study multidomain proteins.
- The synthesis of 1,2-cis-amino containing oligosaccharides toward biological investigation.
- Computational Studies of Glycan Conformations in Glycoproteins
- Architecture and roles of periplasmic adaptor proteins in tripartite efflux assemblies
- In silico Investigation of the PglB Active Site Reveals Transient Catalytic States and Octahedral Metal Ion Coordination.
- Sequence-based protein stabilization in the absence of glycosylation
- Carbohydrate–Protein Interactions: A 3D View by NMR
- Population distribution of flexible molecules from maximum entropy analysis using different priors as background information: application to the Φ, Ψ-conformational space of the α-(1-->2)-linked mannose disaccharide present in N- and O-linked glycoproteins.
- Simultaneous Glycan-Peptide Characterization Using Hydrophilic Interaction Chromatography and Parallel Fragmentation by CID, Higher Energy Collisional Dissociation, and Electron Transfer Dissociation MS Applied to the N-Linked Glycoproteome of Campylobacter jejuni*
- All-atom ensemble modeling to analyze small angle X-ray scattering of glycosylated proteins
- The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates
- Posttranslational modifications of intact proteins detected by NMR spectroscopy: application to glycosylation.
- Mechanisms and principles of N-linked protein glycosylation.
- The Expanding Horizons of Asparagine-Linked Glycosylation
- Cytoplasmic N-Glycosyltransferase of Actinobacillus pleuropneumoniae Is an Inverting Enzyme and Recognizes the NX(S/T) Consensus Sequence*
- Paramagnetic lanthanide tagging for NMR conformational analyses of N-linked oligosaccharides.
- Recent advances in glycoprotein production for structural biology: toward tailored design of glycoforms.
- Protein glycosylation in bacteria: sweeter than ever
- Unexpected reactivity and mechanism of carboxamide activation in bacterial N-linked protein glycosylation
Related papers
- Effect of glycosylation on the protein pattern in 2‐D‐gel electrophoresis
- Defect in N-glycosylation of proteins is tissue-dependent in congenital disorders of glycosylation Ia.
- Producing Biologics with Defined N-Glycosylation in Plants.
- N-Glycosylation of Serum IgG and Total Glycoproteins in MAN1B1 Deficiency.
- Mucin precursors. Identification and analysis of their intracellular processing.
- Altered glycosylation of surface glycoproteins in tumor cells and its clinical application.
- Comparing the glycosylation patterns of recombinant glycoproteins.
- In vitro glycosylation of proteins: an enzymatic approach.
- A novel carbohydrate-deficient glycoprotein syndrome characterized by a deficiency in glucosylation of the dolichol-linked oligosaccharide.