Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX.
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Summary
A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank.
- Type
- article
- Published
- 1983-04-25
- Cited by
- 139
- References
- 0
- Access
- Open access
- OpenAlex
- https://openalex.org/W1966845579
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:8305958
Keywords
Biology, Factor IX, Oligonucleotide, Complementary DNA, clone (Java method)
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Cited by
- The Molecular Genetics of Haemophilia A and B
- Characterization of Genetic Defects of Hemophilia B of Chinese Origin
- Identification of a single nucleotide C-to-T transition and five different deletions in patients with severe hemophilia B.
- Gene cloning based on long oligonucleotide probes.
- Molecular defect in coagulation factor XFriuli results from a substitution of serine for proline at position 343.
- The applications of synthetic oligonucleotides to molecular biology
- The genetics of blood coagulation.
- Intragenic Factor IX restriction site polymorphism in hemophilia B variants.
- Advances in the prenatal diagnosis of hematologic diseases.
- Factor IXAlabama: a point mutation in a clotting protein results in hemophilia B.
- Application of molecular genetics to prenatal diagnosis and carrier detection in the hemophilias: some limitations.
- Functionally important regions of the factor IX gene have a low rate of polymorphism and a high rate of mutation in the dinucleotide CpG.
- Long synthetic oligonucleotide probes for gene analysis.
- Factor XI: structure-function relationships utilizing monoclonal antibodies protein modification, computational chemistry, and rational synthetic peptide design.
- Synthetic oligonucleotide probes deduced from amino acid sequence data. Theoretical and practical considerations.
- Recent advances in the development of coagulation factors and procoagulants for the treatment of hemophilia.
- Molecular cloning of a cDNA encoding human antihaemophilic factor
- Propeptide of human protein C is necessary for gamma-carboxylation.
- The cDNA Sequence of Human Endothelial Cell Multimerin
- Characterization of a rabbit factor IX cDNA.
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