A new generalizable test for detection of mutations affecting Tn10 transposition.
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Summary
A new rapid screen that allows easy detection of transposon or host mutations that affect Tn10 transposition in Escherichia coli is described and successfully used to identify mutations in the terminal sequences of Tn 10.
- Type
- article
- Published
- 1987-06-01
- Cited by
- 48
- References
- 18
- Access
- Open access
- OpenAlex
- https://openalex.org/W1948490957
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:21646129
Keywords
Tn10, Transposable element, Transposition (logic), Biology, Transposase
References
- Experiments in molecular genetics
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- A general method for maximizing the expression of a cloned gene.
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- Genetic organization of transposon Tn10.
- In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage.
- New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition.
- Improved single and multicopy lac-based cloning vectors for protein and operon fusions.
- IS10 transposition is regulated by DNA adenine methylation.
- Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.
- Three promoters near the termini of IS10: pIN, pOUT, and pIII.
- 23 – β-Galactosidase Gene Fusions for Analyzing Gene Expression in Escherichia coli and Yeast
- Essential sites at transposon TnlO termini
Cited by
- A specific class of IS10 transposase mutants are blocked for target site interactions and promote formation of an excised transposon fragment.
- The effect of host‐encoded nucleoid proteins on transposition: H‐NS influences targeting of both IS903 and Tn10
- A practical guide to the construction and use of lac fusions in Escherichia coli.
- Genetic fusions as experimental tools.
- Uses of transposons with emphasis on Tn10.
- Mutagenesis of the IS1 transposase: importance of a His-Arg-Tyr triad for activity
- Recombinaison specifique de site chez les archaea. Implication dans le cycle du virus SSV1 de Sulfolobus shibatae
- Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7.
- Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions.
- Tn10/IS10 transposition is downregulated at the level of transposase expression by the RNA‐binding protein Hfq
- Transposition is modulated by a diverse set of host factors in Escherichia coli and is stimulated by nutritional stress
- Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeast
- Various effects on transposition activity and survival of Escherichia coli cells due to different ELF-MF signals
- The N-terminus of Himar1 mariner transposase mediates multiple activities during transposition
- Measurements of transposition frequency of insertion sequence IS1 by GFP hop-on assay.
- Tn10 transposase mutants with altered transpososome unfolding properties are defective in hairpin formation.
- A bacterial genetic screen identifies functional coding sequences of the insect mariner transposable element Famar1 amplified from the genome of the earwig, Forficula auricularia.
- The three chemical steps of Tn10/IS10 transposition involve repeated utilization of a single active site.
- Extremely low frequency magnetic field exposure affects DnaK and GroEL expression in E. coli cells with impaired heat shock response.
- Development of a new "GFP hop-on assay" system for insertion sequence transposition in Bacillus subtilis 168 using IS4Bsu1 from B. subtilis (natto).
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