Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector
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Summary
The use of a novel suicide vector containing the pir-dependent R6K replicon and the sacB gene of Bacillus subtilis to construct an eae deletion mutant of EPEC is reported, establishing the utility of a new system for the construction of deletion mutations.
- Type
- article
- Published
- 1991-12-01
- Cited by
- 1,126
- References
- 42
- Access
- Open access
- OpenAlex
- https://openalex.org/W1931700765
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:29173547
Keywords
Biology, Mutant, Plasmid, Enteropathogenic Escherichia coli, Escherichia coli
References
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- Etiology of childhood diarrhea in Beijing, China
- A clinicopathologic study of enterocyte-adherent Escherichia coli: a cause of protracted diarrhea in infants.
- A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
- Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria
- Prevalence of Escherichia coli strains with localized, diffuse, and aggregative adherence to HeLa cells in infants with diarrhea and matched controls
- Construction and analysis of TnphoA mutants of enteropathogenic Escherichia coli unable to invade HEp-2 cells
- The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid
- Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa
- Infection of gnotobiotic pigs with an Escherichia coli O157:H7 strain associated with an outbreak of hemorrhagic colitis
- The nucleotide sequence of pACYC184
- ESCHERICHIA COLI STRAINS THAT CAUSE DIARRHŒA BUT DO NOT PRODUCE HEAT-LABILE OR HEAT-STABLE ENTEROTOXINS AND ARE NON-INVASIVE
- Pathogenesis of escherichia coli gastroenteritis in man--another mechanism.
- Detection of Shigella in feces using DNA amplification.
- Gel electrophoresis of proteins: A practical approach
- Identification of invasin: a protein that allows enteric bacteria to penetrate cultured mammalian cells.
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria
- Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K.
- Synthesis of cholera toxin is positively regulated at the transcriptional level by toxR.
Cited by
- Starvation-induced expression of retron-Ec107 and the role of ppGpp in multicopy single-stranded DNA production
- Regulation of the spvR gene of the Salmonella typhimurium virulence plasmid during exponential-phase growth in intracellular salts medium and at stationary phase in L broth.
- Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model.
- Site‐directed mutagenesis of intimin α modulates intimin‐mediated tissue tropism and host specificity
- Co‐ordination of pathogenicity island expression by the BipA GTPase in enteropathogenic Escherichia coli (EPEC)
- Expression of heterologous antigens in Salmonella Typhimurium vaccine vectors using the in vivo-inducible, SPI-2 promoter, ssaG.
- Characterization of the Small Untranslated RNA RyhB and Its Regulon in Vibrio cholerae
- PhoB Regulates Motility, Biofilms, and Cyclic di-GMP in Vibrio cholerae
- Travelers' diarrhea in Nepal: an update on the pathogens and antibiotic resistance.
- A Disulfide Bond in the Membrane Protein IgaA Is Essential for Repression of the RcsCDB System
- Cloning of the RDEC-1 locus of enterocyte effacement (LEE) and functional analysis of the phenotype on HEp-2 cells.
- Mécanismes moléculaires de l'adaptation au cours de 20 000 générations d'évolution expérimentale chez Escherichia coli
- StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor
- Enterohemorrhagic Escherichia coli, EHEC.Microbiological diagnosis, characterisation and clinical bacteriological aspects
- EVALUATION OF THE ROLE OF BarA-UvrY TWO-COMPONENT SYSTEM IN ESCHERICHIA COLI.
- Identification and functional analysis of vibrio vulnificus SmcR, a novel global regulator.
- Identification of the Vibrio vulnificus fexA gene and evaluation of its influence on virulence
- Entry of enteropathogenic Escherichia coli into host cells.
- Tumor-targeted Salmonella typhimurium overexpressing cytosine deaminase: a novel, tumor-selective therapy.
- A pathogenic bacterium triggers epithelial signals to form a functional bacterial receptor that mediates actin pseudopod formation.
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