Homing of a bacterial group II intron with an intron‐encoded protein lacking a recognizable endonuclease domain
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Summary
It is shown that RmInt1 is capable of inserting into a vector containing the DNA spanning the Rm Int1 target site from the genome of S. meliloti and is able to move to its target in a heterologous host (S. medicae).
- Type
- article
- Published
- 2000-03-01
- Cited by
- 62
- References
- 48
- Access
- Open access
- OpenAlex
- https://openalex.org/W1908592049
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:30064080
Keywords
Biology, Homing endonuclease, Intron, Group II intron, Endonuclease
References
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- Efficient DNA transformation of Bradyrhizobium japonicum by electroporation
- Evolution of retroposons by acquisition or deletion of retrovirus-like genes.
- The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus
- A bacterial group II intron encoding reverse transcriptase, maturase, and DNA endonuclease activities: biochemical demonstration of maturase activity and insertion of new genetic information within the intron.
- Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double‐stranded DNA
- Evidence for a group II intron in Escherichia coli inserted into a highly conserved reading frame associated with mobile DNA sequences.
- Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
- Host-Symbiont Interactions : V. THE STRUCTURE OF ACIDIC EXTRACELLULAR POLYSACCHARIDES SECRETED BY RHIZOBIUM LEGUMINOSARUM AND RHIZOBIUM TRIFOLII.
- Characterization and splicing in vivo of a Sinorhizobium meliloti group II intron associated with particular insertion sequences of the IS630‐Tc1/IS3 retroposon superfamily
- A group II intron in a conjugative transposon from the gram-positive bacterium, Clostridium difficile.
- Group II self-splicing introns in bacteria
- Mechanisms of Intron Mobility (*)
- Splicing of a group II intron in a functional transfer gene of Lactococcus lactis
- Mobility of yeast mitochondrial group II introns: engineering a new site specificity and retrohoming via full reverse splicing.
- Retrohoming of a bacterial group II intron: mobility via complete reverse splicing, independent of homologous DNA recombination.
- Complete Sequence of a 184-Kilobase Catabolic Plasmid from Sphingomonas aromaticivorans F199
- Evolutionary relationships among group II intron-encoded proteins and identification of a conserved domain that may be related to maturase function.
- The construction of recA–deficient Rhizobium meliloti and R. leguminosarum strains marked with gusA or luc cassettes for use in risk–assessment studies
- Group II intron mobility in yeast mitochondria: target DNA-primed reverse transcription activity of aI1 and reverse splicing into DNA transposition sites in vitro.
Cited by
- The ins and outs of group II introns.
- Identification and characterization of bacterial class E group II introns.
- Group II Introns: Structure and Catalytic Versatility of Large Natural Ribozymes
- Bacterial group II introns and their association with mobile genetic elements.
- Molecular epidemiology of streptococcus agalactiae : mobile elements as genetic markers.
- Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution
- Diversity of group II introns in the genome of Sinorhizobium meliloti strain 1021: splicing and mobility of RmInt1
- Ectopic transposition of a group II intron in natural bacterial populations
- Structural features in the C‐terminal region of the Sinorhizobium meliloti RmInt1 group II intron‐encoded protein contribute to its maturase and intron DNA‐insertion function
- Group II introns in the bacterial world
- Complete Genome Sequence of the RmInt1 Group II Intronless Sinorhizobium meliloti Strain RMO17
- Retrotransposition strategies of the Lactococcus lactis Ll.LtrB group II intron are dictated by host identity and cellular environment
- Exon sequence requirements for excision in vivo of the bacterial group II intron RmInt1
- Reverse transcriptase and reverse splicing activities encoded by the mobile group II intron cobI1 of fission yeast mitochondrial DNA.
- Structural and Functional Characteristics of Homing Endonucleases
- Splicing of the Sinorhizobium meliloti RmInt1 group II intron provides evidence of retroelement behavior
- An alternative intron-exon pairing scheme implied by unexpected in vitro activities of group II intron RmInt1 from Sinorhizobium meliloti.
- Naturally Occurring Lactococcal Plasmid pAH90 Links Bacteriophage Resistance and Mobility Functions to a Food-Grade Selectable Marker
- DNA target site requirements for homing in vivo of a bacterial group II intron encoding a protein lacking the DNA endonuclease domain.
- Bacteria and Archaea Group II introns: additional mobile genetic elements in the environment.
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