Tear film proteins deposited on high water content contact lenses identified with two‐dimensional gel electrophoresis and mass spectrometry
Explore this paper's citation graph
Summary
This pilot study has separated proteins with 2D gel electrophoresis prior to the conventional mass spectrometry (MS) in order to analyse the deposited proteins on hydrogel CLs from myopic patients.
- Type
- article
- Published
- 2015-11-01
- Cited by
- 4
- References
- 22
- Access
- Open access
- OpenAlex
- https://openalex.org/W1903962407
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:30943240
Keywords
Mass spectrometry, Chromatography, Two-dimensional gel electrophoresis, Electrophoresis, Chemistry
References
- Quantitation of total protein deposits on contact lenses by means of amino acid analysis.
- The normal tear film.
- Investigation of the human tear film proteome using multiple proteomic approaches
- Protein changes in the retina following experimental retinal detachment in rabbits
- Effect of contact lenses on the protein composition in tear film: a ProteinChip study
- Mass Spectrometry-Based Proteomic Analyses in Contact Lens-Related Dry Eye
- Probability-based protein identification by searching sequence databases using mass spectrometry data
- Attempting to distinguish between endogenous and contaminating cytokeratins in a corneal proteomic study
- Antimicrobial Compounds in Tears
- Method development for quantification of five tear proteins using selected reaction monitoring (SRM) mass spectrometry.
- Characterization of human tear proteome using multiple proteomic analysis techniques.
- Matrix-assisted laser desorption ionization mass spectrometry detection of proteins adsorbed in vivo onto contact lenses.
- Improved silver staining protocols for high sensitivity protein identification using matrix‐assisted laser desorption/ionization‐time of flight analysis
- Laboratory findings in tear fluid analysis.
- Factors influencing bacterial adhesion to contact lenses
- Quantification of individual proteins in silicone hydrogel contact lens deposits
- Proteomic analysis of protein deposits on worn daily wear silicone hydrogel contact lenses
- Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors
- Protein deposition on contact lenses: the past, the present, and the future.
- Giant papillary conjunctivitis.
Cited by
- A Review of Techniques to Measure Protein Sorption to Soft Contact Lenses
- Functional Hydrogels and Their Application in Drug Delivery, Biosensors, and Tissue Engineering
- Tear Proteomics of Children and Young Adult Soft Contact Lens, Orthokeratology and Spectacle Wearers – A Pilot Study
- A review of the last 10 years regarding protein and lipid deposition on soft contact lenses.
Related papers
- Movement of Electrophoretic Particles between Two Electrodes in Electrophoretic Image Display System
- Novel Color Electrophoretic Imaging Display Based on Movement of Particles Using Two Driving Electrodes
- High-pressure liquid chromatographic analysis of drugs in biological fluids. IV. Determination of clofibrinic acid.
- Automated liquid membrane extraction for high-performance liquid chromatography of Ropivacaine metabolites in urine.
- The use of bonded-phase extraction columns for rapid sample preparation of benzodiazepines and metabolites from serum for HPLC analysis.
- Application of totally automated on-line sample clean up system for extraction and high-performance liquid chromatography separation of peptide leukotrienes.
- Starch-gel electrophoresis of rat-serum proteins.
- Micro-electrophoresis apparatus