Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast.
Explore this paper's citation graph
Summary
A novel multienzyme approach was used to generate a set of highly representative genomic libraries from S. cerevisiae and a unique host strain was created that contains three easily assayed reporter genes, each under the control of a different inducible promoter.
- Type
- article
- Published
- 1996-12-01
- Cited by
- 2,892
- References
- 36
- Access
- Open access
- OpenAlex
- https://openalex.org/W1903792504
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:19094968
Keywords
Biology, False positive paradox, Saccharomyces cerevisiae, Genetics, Plasmid
References
- The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
- Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
- Regulation of RAD53 by the ATM-Like Kinases MEC1 and TEL1 in Yeast Cell Cycle Checkpoint Pathways
- Yeast chromosome III: new gene functions.
- Elimination of false positives that arise in using the two-hybrid system.
- A family of proteins containing a conserved domain that mediates interaction with the yeast SNF1 protein kinase complex.
- Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
- A GAL family of upstream activating sequences in yeast: roles in both induction and repression of transcription.
- The complete DNA sequence of yeast chromosome III
- Methods in yeast genetics
- Experiments in molecular genetics
- Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.
- The MET2 gene of Saccharomyces cerevisiae: molecular cloning and nucleotide sequence.
- The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.
- A novel genetic system to detect proteinprotein interactions
- Interactions between three common subunits of yeast RNA polymerases I and III.
- Coordinated regulation of a set of genes by glucose and calcium ionophores in mammalian cells
- Mono- through hexanucleotide composition of the sense strand of yeast DNA: a Markov chain analysis.
- A protein kinase substrate identified by the two-hybrid system.
- Construction of an improved host strain for two hybrid screening.
Cited by
- Construction of a new model system in Saccharomyces cerevisiae to study YopO and identify its targets
- Multiple roles for UNC-53/NAV2 in cell migration, trafficking and innate immunity in Caenorhabditis elegans
- Homomeric interaction of the mouse Rad52 protein
- Aut2p and Aut7p, two novel microtubule‐associated proteins are essential for delivery of autophagic vesicles to the vacuole
- Interaction of Kar2p and Sls1p Is Required for Efficient Co-translational Translocation of Secreted Proteins in the YeastYarrowia lipolytica *
- Sec24 Proteins and Sorting at the Endoplasmic Reticulum*
- Tip60 Is a Nuclear Hormone Receptor Coactivator*
- Sls1p Stimulates Sec63p-Mediated Activation of Kar2p in a Conformation-Dependent Manner in the Yeast Endoplasmic Reticulum
- NudE-L, a novel Lis1-interacting protein, belongs to a family of vertebrate coiled-coil proteins.
- Site‐directed mutagenesis of intimin α modulates intimin‐mediated tissue tropism and host specificity
- N-Terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8.
- A discrete amino terminal domain of Kv1.5 and Kv1.4 potassium channels interacts with the spectrin repeats of α‐actinin‐2
- Targeting of the Yeast Ty5 Retrotransposon to Silent Chromatin Is Mediated by Interactions between Integrase and Sir4p
- Role of Deltex-1 as a Transcriptional Regulator Downstream of the Notch Receptor*
- Functions of Vrp1p in cytokinesis and actin patches are distinct and neither requires a WH2/V domain
- Functional analysis of the enteropathogenic Escherichia coli type III secretion system chaperone CesT identifies domains that mediate substrate interactions
- A Cysteine-Rich Extracellular Protein, LAT52, Interacts with the Extracellular Domain of the Pollen Receptor Kinase LePRK2 Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.003103.
- Protein Phosphatase-1 Binding to Scd5p Is Important for Regulation of Actin Organization and Endocytosis in Yeast*
- Controlling integration specificity of a yeast retrotransposon
- Trl-GAGA directly interacts with lola like and both are part of the repressive complex of Polycomb group of genes.
Related papers
- Successful spread of mcr-1-bearing IncX4 plasmids is associated with variant in replication protein of IncX4 plasmids
- Conjugation efficiency depends on intra and intercellular interactions between distinct plasmids: Plasmids promote the immigration of other plasmids but repress co-colonizing plasmids.
- False positives and false negatives in genome scans.
- Where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges
- Retrotransfer of IncP1‐like plasmids from aquatic bacteria
- Impact of plasmid interactions with the chromosome and other plasmids on the spread of antibiotic resistance.
- Positive epistasis between co-infecting plasmids promotes plasmid survival in bacterial populations