CRISPR Primer Designer: Design primers for knockout and chromosome imaging CRISPR-Cas system.
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Summary
The CRISPR Primer Designer program has a user-friendly interface, can analyze the BLAST results by using multiple parameters, score for each candidate spacer, and generate the primers when using a certain plasmid.
- Type
- article
- Published
- 2015-07-01
- Cited by
- 41
- References
- 22
- OpenAlex
- https://openalex.org/W1802315863
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:7033058
Keywords
CRISPR, Primer (cosmetics), Genome editing, Computational biology, Cas9
References
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- A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
- Targeted mutagenesis in rice using CRISPR-Cas system
- Multiplex Genome Engineering Using CRISPR/Cas Systems
- Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System
- Efficient genome editing in plants using a CRISPR/Cas system
- High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
- Application of the CRISPR-Cas system for efficient genome engineering in plants.
- Cas9 as a versatile tool for engineering biology
- CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
- ZFN, TALEN and CRISPR/Cas-based methods for genome engineering
- CasOT: a genome-wide Cas9/gRNA off-target searching tool
- Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
- Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
Cited by
- A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana
- Use of designer nucleases for targeted gene and genome editing in plants.
- CRISPR/Cas9: A powerful tool for crop genome editing
- Generation of inheritable and “transgene clean” targeted genome-modified rice in later generations using the CRISPR/Cas9 system
- Targeted Gene Manipulation in Plants Using the CRISPR/Cas Technology.
- The potential of the mevalonate pathway for enhanced isoprenoid production.
- High-throughput CRISPRi phenotyping in Streptococcus pneumoniae identifies new essential genes involved in cell wall synthesis and competence development
- RNA-guided Genome Editing Tool CRISPR-Cas9: Its Applications and Achievements in Model and Crop Plants
- CRISPR-Cas9 and CRISPR-Cpf1 mediated targeting of a stomatal developmental gene EPFL9 in rice
- CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus
- Rice Interploidy Crosses Disrupt Epigenetic Regulation, Gene Expression, and Seed Development.
- Multigene editing via CRISPR/Cas9 guided by a single-sgRNA seed in Arabidopsis.
- SDG721 and SDG705 are required for rice growth.
- Genome editing technologies and their applications in crop improvement
- SIP1 participates in regulation of flowering time in rice by recruiting OsTrx1 to Ehd1
- Development and Validation of an Effective CRISPR/Cas9 Vector for Efficiently Isolating Positive Transformants and Transgene-Free Mutants in a Wide Range of Plant Species
- CRISPR Interference for Rapid Knockdown of Essential Cell Cycle Genes in Lactobacillus plantarum
- Transcriptional Knockdown in Pneumococci Using CRISPR Interference.
- The rice PLATZ protein SHORT GRAIN6 determines grain size by regulating spikelet hull cell division.
- [Design of Guide RNA for CRISPR/Cas Plant Genome Editing].
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