Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers
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Summary
It is shown that intrinsically disordered, repeat proteins can be designed to exhibit tunable lower or upper critical solution temperature transitions in physiological solutions and that mutation of key residues at the repeat level abolishes phase behaviour or encodes an orthogonal transition.
- Type
- article
- Published
- 2015-09-21
- Cited by
- 394
- References
- 57
- Access
- Open access
- OpenAlex
- https://openalex.org/W1800445960
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:10455750
Keywords
Upper critical solution temperature, Lower critical solution temperature, Polymer, Sequence (biology), Proteome
References
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- First Example of a Universal and Cost-Effective Approach: Polymers with Tunable Upper Critical Solution Temperature in Water and Electrolyte Solution
- Proline Periodicity Modulates the Self-assembly Properties of Elastin-like Polypeptides*
- Elastin-like protein matrix reinforced with collagen microfibers for soft tissue repair
- Getting RNA and protein in phase.
- Unmasking the roles of N- and C-terminal flanking sequences from exon 1 of huntingtin as modulators of polyglutamine aggregation
- Introducing protein intrinsic disorder.
- The Protein-Folding Problem, 50 Years On
- Multiblock Polymers: Panacea or Pandora’s Box?
- Triple stimulus-responsive polypeptide nanoparticles that enhance intratumoral spatial distribution
- Self-assembling elastin-like peptides growth factor chimeric nanoparticles for the treatment of chronic wounds
Cited by
- Biomolecular Chemistry in Liquid Phase Separated Compartments
- Protein polymers: Encoding phase transitions.
- Noncovalent Modulation of the Inverse Temperature Transition and Self-Assembly of Elastin-b-Collagen-like Peptide Bioconjugates
- Phase separation in biology; functional organization of a higher order
- Role of Bassoon and Piccolo in Assembly and Molecular Organization of the Active Zone
- Mechanisms and Consequences of Macromolecular Phase Separation.
- Effects of Crowding and Environment on the Evolution of Conformational Ensembles of the Multi-Stimuli-Responsive Intrinsically Disordered Protein, Rec1-Resilin: A Small-Angle Scattering Investigation.
- A glass menagerie of low complexity sequences
- Aggregation behaviour of biohybrid microgels from elastin-like recombinamers.
- Experimental models for dynamic compartmentalization of biomolecules in liquid organelles: Reversible formation and partitioning in aqueous biphasic systems.
- Rapid Brownian Motion Primes Ultrafast Reconstruction of Intrinsically Disordered Phe-Gly Repeats Inside the Nuclear Pore Complex
- Non-covalent interactions in controlling pH-responsive behaviors of self-assembled nanosystems
- Are aberrant phase transitions a driver of cellular aging?
- Orientation and organization of the presynaptic active zone protein Bassoon: from the Golgi to the synapse
- RNA-Based Coacervates as a Model for Membraneless Organelles: Formation, Properties, and Interfacial Liposome Assembly.
- Modular peptides from the thermoplastic squid sucker ring teeth form amyloid-like cross-β supramolecular networks.
- Random-phase-approximation theory for sequence-dependent, biologically functional liquid-liquid phase separation of intrinsically disordered proteins
- Liquid-liquid phase separation of a monoclonal antibody at low ionic strength: Influence of anion charge and concentration.
- Molecular basis of cooperativity in pH-triggered supramolecular self-assembly
- Reversible Monolayer/Spheroid Cell Culture Switching by UCST-Type Thermoresponsive Ureido Polymers.
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