Generation of a stable packaging cell line producing high-titer PPT-deleted integration-deficient lentiviral vectors
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Summary
The development of the first tetracycline-inducible stable IDLV packaging cell line comprising the D64E integrase mutant and the VSV-G envelope protein is described, which will significantly facilitate IDLV-based basic science research, as well as preclinical and clinical applications.
- Type
- article
- Published
- 2015-07-22
- Cited by
- 16
- References
- 72
- Access
- Open access
- OpenAlex
- https://openalex.org/W1791343928
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:10961597
Keywords
Transfection, Transduction (biophysics), Cell culture, Titer, Plasmid
References
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- Integrase-defective lentiviral vectors: progress and applications
- Genomic instability and myelodysplasia with monosomy 7 consequent to EVI1 activation after gene therapy for chronic granulomatous disease
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- A foundation for universal T-cell based immunotherapy: T cells engineered to express a CD19-specific chimeric-antigen-receptor and eliminate expression of endogenous TCR.
- Vaccines delivered by integration-deficient lentiviral vectors targeting dendritic cells induces strong antigen-specific immunity
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- Integrase-defective lentiviral vectors as a delivery platform for targeted modification of adenosine deaminase locus.
- Development and use of SIV-based Integrase defective lentiviral vector for immunization
- Whole transcriptome characterization of aberrant splicing events induced by lentiviral vector integrations.
- Transduction of Human Antigen-Presenting Cells with Integrase-Defective Lentiviral Vector Enables Functional Expansion of Primed Antigen-Specific CD8+ T Cells
- Efficient gene expression from integration-deficient lentiviral vectors in the spinal cord
- Nonintegrating Lentivector Vaccines Stimulate Prolonged T-Cell and Antibody Responses and Are Effective in Tumor Therapy
Cited by
- Production of lentiviral vectors
- Progress and challenges in viral vector manufacturing.
- Experimental Methods to Preserve Male Fertility and Treat Male Infertility
- Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS
- In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models.
- Deficiency of Functional Iron-Sulfur Domains in ABCE1 Inhibits the Proliferation and Migration of Lung Adenocarcinomas By Regulating the Biogenesis of Beta-Actin In Vitro
- Lentiviral vector packaging cell line development using genome editing to target optimal loci discovered by high throughput DNA barcoding
- Targeted in vivo knock-in of human alpha-1-antitrypsin cDNA using adenoviral delivery of CRISPR/Cas9
- Superior lentiviral vectors designed for BSL-0 environment abolish vector mobilization.
- Progress and Perspectives in the Development of Lentiviral Vector Producer Cells
- The Old and the New: Prospects for Non-Integrating Lentiviral Vector Technology
- Novel immortalized human vocal fold epithelial cell line: In vitro tool for mucosal biology
- The transformative potential of HSC gene therapy as a genetic medicine
- Production of Lentiviral Vectors Using a HEK-293 Producer Cell Line and Advanced Perfusion Processing
- All-in-one IQ toggle switches with high versatilities for fine-tuning of transgene expression in mammalian cells and tissues
- Application Advances of Lentiviral Vectors: From Gene Therapy to Vaccine Development.
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