Detection of Gene Expression in Genetically Engineered Microorganisms and Natural Phytoplankton Populations in the Marine Environment by mRNA Analysis
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Summary
The first report of gene expression in natural populations by mRNA isolation and probing is reported, using this method to detect expression of the ribulose-1,5-bisphosphate carboxylase large-subunit gene in Synechococcus cultures and natural phytoplankton populations in the Dry Tortugas, Florida.
- Type
- article
- Published
- 1991-06-01
- Cited by
- 68
- References
- 31
- Access
- Open access
- OpenAlex
- https://openalex.org/W1790109529
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:7475730
Keywords
Biology, Gene expression, Gene, RNA, RNA extraction
References
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- Antiserum to Nitrogenase Generated from an Amplified DNA Fragment from Natural Populations of Trichodesmium spp
- Broad-host-range IncP-4 plasmid R1162: effects of deletions and insertions on plasmid maintenance and host range
- Effects of Hg2+, CH3-Hg+, and Temperature on the Expression of Mercury Resistance Genes in Environmental Bacteria
- Use of Hoechst Dyes 33258 and 33342 for Enumeration of Attached and Planktonic Bacteria
- Basis for Diel Variation in Nitrogenase Activity in the Marine Planktonic Cyanobacterium Trichodesmium thiebautii
- Natural plasmid transformation in a high-frequency-of-transformation marine Vibrio strain
- Transcriptional and post-transcriptional regulation of ribulose 1,5-bisphosphate carboxylase gene expression in light- and dark-grown amaranth cotyledons.
- Phytochrome-mediated regulation of two mRNAs, encoded by nuclei and chloroplasts of ribulose-1,5-bisphosphate carboxylase/oxygenase.
- Chlorophyll a Determination: Improvements in Methodology
- Inverted repeat of Olisthodiscus luteus chloroplast DNA contains genes for both subunits of ribulose-1,5-bisphosphate carboxylase and the 32,000-dalton Q(B) protein: Phylogenetic implications.
- Coordinate light‐induction of two mRNAs, encoded in nuclei and chloroplasts, of ribulose 1,5‐bisphosphate carboxylase/oxygenase
- Molecular cloning and sequence analysis of the cyanobacterial gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase.
- The cyclic synthesis of the nitrogenase of Synechococcus RF-1 and its control at the transcription level
- Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.
- Transcriptional regulation of genes for plant-type ribulose-1,5-bisphosphate carboxylase/oxygenase in the photosynthetic bacterium, Chromatium vinosum.
- The gene for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is located close to the gene for the large subunit in the cyanobacterium Anacystis nidulans 6301.
- Light regulates the gene expression of ribulosebisphosphate carboxylase at the levels of transcription and gene dosage in greening pea leaves
- Molecular and cellular regulation of autotrophic carbon dioxide fixation in microorganisms.
- Expression and assembly of active cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase in Escherichia coli containing stoichiometric amounts of large and small subunits.
Cited by
- Magnetic capture-hybridization method for purification and probing of mRNA for neutral protease of Bacillus cereus.
- Stable isotope fractionation analysis as a tool to monitor biodegradation in contaminated acquifers.
- Gene Expression in Two Cyanobacteria, Freshwater Synechococcus sp. PCC 7942 and Oceanic Synechococcus sp. WH 7803, in response to ammonium, nitrate or iron
- MEASUREMENT OF IN SITU EXPRESSION OF PROTEORHODOPSIN GENES AT THE NORTH PACIFIC CENTRAL GYRE STATION ALOHA
- Use of viral pathogens and indicators to differentiate between human and non-human fecal contamination in a microbial source tracking comparison study.
- Gene Expression per Gene Dose, a Specific Measure of Gene Expression in Aquatic Microorganisms
- Enteroviruses detected by reverse transcriptase polymerase chain reaction from the coastal waters of Santa Monica Bay, California: low correlation to bacterial indicator levels
- merA gene expression in aquatic environments measured by mRNA production and Hg(II) volatilization
- Measurement of rRNA Variations in Natural Communities of Microorganisms on the Southeastern U.S. Continental Shelf
- Conjugal Gene Transfer to Aquatic Bacteria Detected by the Generation of a New Phenotype
- Diversity of the ribulose bisphosphate carboxylase/oxygenase form I gene (rbcL) in natural phytoplankton communities
- In Situ, Real-Time Catabolic Gene Expression: Extraction and Characterization of Naphthalene Dioxygenase mRNA Transcripts from Groundwater
- Simple method of concentrating enteroviruses and hepatitis A virus from sewage and ocean water for rapid detection by reverse transcriptase-polymerase chain reaction
- Diel Rhythms in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Glutamine Synthetase Gene Expression in a Natural Population of Marine Picoplanktonic Cyanobacteria (Synechococcusspp.)
- Detection of hepatitis A virus, rotavirus, and enterovirus in naturally contaminated shellfish and sediment by reverse transcription-seminested PCR
- Rubisco Expression in the Dinoflagellate Symbiodinium sp. Is Influenced by Both Photoperiod and Endosymbiotic Lifestyle
- Bacterial Endophytes: Potential Role in Developing Sustainable Systems of Crop Production
- Ribulose-Bisphosphate Carboxylase Gene Expression in Subtropical Marine Phytoplankton Populations
- Methods of microbial identification, tracking and monitoring of function
- Conventional and molecular methods for understanding probiotic bacteria functionality in gastrointestinal tracts.
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