Preparation, culture, and immortalization of mouse embryonic fibroblasts.
Explore this paper's citation graph
Summary
Detailed protocols regarding isolation of mouse embryonic fibroblasts (MEFs) from mouse embryos, culture of MEFs, and immortalize primary MEFs into stable cell cultures with a permanent growth feature from wild-type and mutant mouse embryos are described.
- Type
- article
- Published
- 2005-05-01
- Cited by
- 329
- References
- 7
- OpenAlex
- https://openalex.org/W1691160266
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:6199092
Keywords
Embryonic stem cell, Biology, Transgene, Cell biology, Genetically modified mouse
References
- Deletion of the Cancer-amplified Coactivator AIB3 Results in Defective Placentation and Embryonic Lethality*
- Development of 3T3‐like lines from Balb/c mouse embryo cultures: Transformation susceptibility to SV40
- Cell growth and the initiation of transformation by SV40.
- Selective phosphorylations of the SRC-3/AIB1 coactivator integrate genomic reponses to multiple cellular signaling pathways.
- QUANTITATIVE STUDIES OF THE GROWTH OF MOUSE EMBRYO CELLS IN CULTURE AND THEIR DEVELOPMENT INTO ESTABLISHED LINES
- Derivation of embryonic stem cell lines.
Cited by
- High throughput automated chromatin immunoprecipitation as a platform for drug screening and antibody validation,
- ALKBH4-dependent demethylation of actin regulates actomyosin dynamics
- Notch signaling functions in retinal pericyte survival.
- Conditional inactivation of PDCD2 induces p53 activation and cell cycle arrest
- Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B
- The Role of Redox-dependent Reactions with Kras Cysteine 118 in Tumorigenesis
- Investigating genotype-phenotype correlations and potential therapies for laminopathies
- Mechanosensitive pannexin-1 channels mediate microvascular metastatic cell survival
- Alk2 Regulates Early Chondrogenic Fate in Fibrodysplasia Ossificans Progressiva Heterotopic Endochondral Ossification
- Manipulating Embryonic Neural Precursor Cells for Therapeutic Transplantation into a Rat Model of Neuropathic Pain
- Funktionelle Charakterisierung des Two-Pore-Loop-Kanals 2 (TPC2) im endolysosomalen System
- Serine/Threonine Protein Phosphatase 5 - a Double-edged Sword - in the Progression towards Diabetes
- Cdk8 deletion in the ApcMin murine tumour model represses EZH2 activity and accelerates tumourigenesis
- Label-Free Protein-RNA Interactome Analysis Identifies Khsrp Signaling Downstream of the p38/Mk2 Kinase Complex as a Critical Modulator of Cell Cycle Progression
- Murine Norovirus: Propagation, Quantification and Genetic Manipulation
- Primer retention owing to the absence of RNase H1 is catastrophic for mitochondrial DNA replication
- DESIGN, SYNTHESIS, AND APPLICATION OF THE TRIMETHOPRIM-BASED CHEMICAL TAG FOR LIVE CELL IMAGING
- The Annexin A2/p11 complex is required for efficient invasion of Salmonella Typhimurium in epithelial cells
- Cholesterol Regulates the Endoplasmic Reticulum Exit of the Major Membrane Protein P0 Required for Peripheral Myelin Compaction
- Gsta4 Null Mouse Embryonic Fibroblasts Exhibit Enhanced Sensitivity to Oxidants: Role of 4-Hydroxynonenal in Oxidant Toxicity
Related papers
- Early Changes in Behavior Deficits, Amyloid β-42 Deposits and MAPK Activation in Doubly Transgenic Mice Co-expressing NSE-Controlled Human Mutant PS2 and APPsw
- Development and Characterization of Thy*IκBα-SI Transgenic Mice
- SIRT1 transgenic mice show phenotypes resembling calorie restriction
- Transgenic mice: beyond the knockout.
- [Transgenic mice in basic research].
- Transgenic mice models in autoimmunity--discussion.
- In vivo expression of a conditional TGF-beta1 transgene: no evidence for TGF-beta1 transgene expression in SM22alpha-tTA transgenic mice.
- Gene expression profile in liver of hB1F transgenic mice.
- Principles for the use of in vivo transgene techniques: overview and an introductory practical guide for the selection of tetracycline-controlled transgenic mice.
- Endothelium‐specific Cre recombinase activity in flk‐1‐Cre transgenic mice