Optimized routine flow cytometric enumeration of heterotrophic flagellates using SYBR Green I
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Summary
Evaluated simplification of some steps of the FC protocol, seeking the best compromise between precision and the quality of distinction of HF from bacteria and phytoplankton in the cytograms, found that a flow rate of 120–220 µL min−1 without using a syringe‐pump enhanced machine modification, and running times of 8–10 min allowed enumeration of HF even at values below 102 cells mL−1.
- Type
- article
- Published
- 2011-08-01
- Cited by
- 107
- References
- 48
- Access
- Open access
- OpenAlex
- https://openalex.org/W1689648552
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:94560811
Keywords
Enumeration, Staining, Heterotroph, Flow cytometry, Bacteria
References
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- Rapid, automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting
- Comparison of Blue Nucleic Acid Dyes for Flow Cytometric Enumeration of Bacteria in Aquatic Systems
- Community heterogeneity and single-cell digestive activity of estuarine heterotrophic nanoflagellates assessed using lysotracker and flow cytometry.
- Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analysis of marine prokaryotes
- Fixation, Counting, and Manipulation of Heterotrophic Nanoflagellates
- Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13
- Flow Cytometric Analysis of Coastal Lagoon Bacterioplankton and Picophytoplankton: Fixation and Storage Effects
- Analysis of nanoplankton community structure using flow sorting and molecular techniques.
- Digestion of bacterial macromolecules by a mixotrophic flagellate, Ochromonas sp., compared with that by two heterotrophic flagellates, Spumella pudica and Bodo saltans
- Assimilation efficiency of Vibrio bacterial protein biomass by the flagellate Pteridomonas : assessment using flow cytometric sorting.
- Syringe pumped high speed flow cytometry of oceanic phytoplankton
- Grazing impact of different heterotrophic nanoflagellates on eukaryotic (Ostreococcus tauri) and prokaryotic picoautotrophs (Prochlorococcus and Synechococcus).
- Capturing diversity of marine heterotrophic protists: one cell at a time
- Distribution and abundance of uncultured heterotrophic flagellates in the world oceans.
- The use of DAPI for identifying and counting aquatic microflora1
- Bacterivory and herbivory: Key roles of phagotrophic protists in pelagic food webs
- Practical Flow Cytometry, 4th Edition
- Simultaneous Fluorescence In Situ Hybridization of mRNA and rRNA in Environmental Bacteria
- DNA damage caused by common cytological fixatives.
Cited by
- Global abundance of planktonic heterotrophic protists in the deep ocean
- Combined effects of zooplankton grazing and dispersal on the diversity and assembly mechanisms of bacterial metacommunities.
- Environmental microbiology as a mosaic of explored ecosystems and issues
- Harmful and parasitic unicellular eukaryotes persist in a shallow lake under reconstruction (L. Karla, Greece)
- Population-specific shifts in viral and microbial abundance within a cryptic upwelling
- Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean.
- Microbial food web dynamics in response to a Saharan dust event: results from a mesocosm study in the oligotrophic Mediterranean Sea
- Microbial food web dynamics during spring phytoplankton blooms in the naturally iron-fertilized Kerguelen area (Southern Ocean)
- Seasonal dynamics and stoichiometry of the planktonic community in the NW Mediterranean Sea: a 3D modeling approach
- Metabolic state along a summer north- south transect near the Antarctic Peninsula: a size spectra approach
- Phylogenetic and structural response of heterotrophic bacteria to dissolved organic matter of different chemical composition in a continuous culture study.
- Changes in Microbial Plankton Assemblages Induced by Mesoscale Oceanographic Features in the Northern Gulf of Mexico
- Ultra- and microplankton assemblages as indicators of trophic status in a Mediterranean lagoon
- Structure of microbial communities in phosphorus-limited estuaries along the eastern Adriatic coast
- Pelagic microbial heterotrophy in response to a highly productive bloom of Phaeocystis antarctica in the Amundsen Sea Polynya, Antarctica
- Application of flow cytometry to enumerate small plankton
- A light-induced shortcut in the planktonic microbial loop
- Composition of diatom communities and their contribution to plankton biomass in the naturally iron-fertilized region of Kerguelen in the Southern Ocean.
- Synechococcus in the Atlantic Gateway to the Arctic Ocean
- Implementing and Innovating Marine Monitoring Approaches for Assessing Marine Environmental Status
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