Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader.
Explore this paper's citation graph
Summary
This method is reliable and efficient for evaluating the potency of pro-oxidants and can be used to evaluate the efficacy of antioxidants against OS in cells, especially when used in conjunction with a fluorescent microplate reader.
- Type
- article
- Published
- 1999-09-01
- Cited by
- 2,373
- References
- 24
- OpenAlex
- https://openalex.org/W1541649582
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:29803768
Keywords
Dichlorofluorescein, Chemistry, Peroxynitrite, Fluorescence, Oxidative stress
References
- Free Radicals in Biology
- Natural antioxidants in human health and disease
- Free radical initiators as source of water- or lipid-soluble peroxyl radicals.
- Measuring free radical reactions in vivo.
- Chemical biology of nitric oxide: regulation and protective and toxic mechanisms.
- Production of hydroxyl radicals from the simultaneous generation of superoxide and nitric oxide.
- Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
- Dopamine Neurotoxicity: Inhibition of Mitochondrial Respiration
- Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay.
- Nitric oxide protects against cellular damage and cytotoxicity from reactive oxygen species.
- Coupling of Dopamine Oxidation (Monoamine Oxidase Activity) to Glutathione Oxidation Via the Generation of Hydrogen Peroxide in Rat Brain Homogenates
- THE FLUOROMETRIC ANALYSIS OF ULTRAMICRO QUANTITIES OF HYDROGEN PEROXIDE.
- Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
- Bright and dark sides of nitric oxide in ischemic brain injury.
- A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compounds
- Monitoring intracellular nitric oxide formation by dichlorofluorescin in neuronal cells.
- Autoxidation versus covalent binding of quinones as the mechanism of toxicity of dopamine, 6-hydroxydopamine, and related compounds toward C1300 neuroblastoma cells in vitro.
- Oxidation of 2',7'-dichlorofluorescin by peroxynitrite.
- Nitric oxide in the nervous system.
- Spectrophotometric assay for total peroxyl radical-trapping antioxidant potential in human serum.
Cited by
- Reactive oxygen species are crucial for hydroxychavicol toxicity toward KB epithelial cells
- A link between hyperbilirubinemia, oxidative stress and injury to neocortical synaptosomes.
- Mouse recombinant leptin protects human hepatoma HepG2 against apoptosis, TNF-alpha response and oxidative stress induced by the hepatotoxin-ethanol.
- Reactive Oxygen Species and Dopamine Receptor Function in Essential Hypertension
- Protective Effect of Memantine Against Doxorubicin Toxicity in Primary Neuronal Cell Cultures: Influence a Development Stage
- Anti-oxidative and anti-inflammatory effects of aerial parts of Rumex japonicus Houtt. in RAW 264.7 cells
- Methylglyoxal augments intracellular oxidative stress in human aortic endothelial cells
- p66SHC-mediated mitochondrial dysfunction in renal proximal tubule cells during oxidative injury.
- The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture
- Aerosolized ZnO nanoparticles induce toxicity in alveolar type II epithelial cells at the air-liquid interface.
- Effect of Grape Seed Proanthocyanidin Extracts on Methylmercury-Induced Neurotoxicity in Rats
- A polyphenol-enriched cocoa extract reduces free radicals produced by mycotoxins.
- Cytotoksyczność i genotoksyczność soku z ziemniaka
- Protective effect of marine algae phlorotannins against AAPH-induced oxidative stress in zebrafish embryo.
- Methods for studying oxidative regulation of protein kinase C.
- ATP-citrate lyase is essential for macrophage inflammatory response.
- Evaluation of Antigenotoxic Effects of Plant Flavonoids Quercetin and Rutin on HepG2 Cells
- Cytotoxicity of TiO2 nanoparticles towards freshwater sediment microorganisms at low exposure concentrations.
- Influence of peptides-phenolics interaction on the antioxidant profile of protein hydrolysates from Brassica napus.
- Systemically Delivered Adipose Stromal Vascular Fraction Cells Disseminate to Peripheral Artery Walls and Reduce Vasomotor Tone Through a CD11b+ Cell‐Dependent Mechanism
Related papers
- Oxidation of 2',7'-dichlorofluorescin by peroxynitrite.
- Drugs modulating the biological effects of peroxynitrite and related nitrogen species
- A highly selective fluorescent probe for the detection and imaging of peroxynitrite in living cells.
- The critical role of reactive nitrogen species in lung ischemia reperfusion injury
- Protection against peroxynitrite‐dependent tyrosine nitration and α 1‐antiproteinase inactivation by oxidized and reduced lipoic acid
- A highly selective water-soluble optical probe for endogenous peroxynitrite.
- Peroxynitrite Formation in Nitric Oxide- Exposed Submitochondrial Particles: Detection and Quantitation by a Boronic Acid- Based Probe
- Dose dependent effects of reactive oxygen and nitrogen species on the function of neuronal nitric oxide synthase.
- Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species.