Recombination-mediated PCR-directed plasmid construction in vivo in yeast.
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Summary
The technique of PCR-directed recombination in Saccharomyces cerevisiae is extended to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites.
- Type
- article
- Published
- 1997-01-15
- Cited by
- 531
- References
- 13
- Access
- Open access
- OpenAlex
- https://openalex.org/W1517699507
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:526779
Keywords
Biology, Plasmid, Saccharomyces cerevisiae, Homologous recombination, Genetics
References
- Methods in yeast genetics
- Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
- A rapid and reliable DNA preparation method for screening a large number of yeast clones by polymerase chain reaction.
- Micro-homology mediated PCR targeting in Saccharomyces cerevisiae.
- Plasmid construction by homologous recombination in yeast.
- A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.
- Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling
- Analysis of the localization of STE6/CFTR chimeras in a Saccharomyces cerevisiae model for the cystic fibrosis defect CFTRΔF508
Cited by
- PCR-directed in vivo plasmid construction using homologous recombination in baker's yeast.
- Topological and mutational analysis of Saccharomyces cerevisiae Ste14p, founding member of the isoprenylcysteine carboxyl methyltransferase family.
- CDG‐IL: An infant with a novel mutation in the ALG9 gene and additional phenotypic features
- Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae.
- Proteolytic processing of certain CaaX motifs can occur in the absence of the Rce1p and Ste24p CaaX proteases
- Detection of replication origins using comparative genomics and recombinational ARS assay.
- Toxoplasma gondii Rhoptry Kinase ROP16 Activates STAT3 and STAT6 Resulting in Cytokine Inhibition and Arginase-1-Dependent Growth Control
- Rapid and reliable DNA assembly via ligase cycling reaction.
- The C-terminal region of the human p23 chaperone modulates its structure and function.
- Kar5p Is Required for Multiple Functions in Both Inner and Outer Nuclear Envelope Fusion in Saccharomyces cerevisiae
- Genome Mining of Streptomyces sp. Tü 6176: Characterization of the Nataxazole Biosynthesis Pathway
- A method to capture large DNA fragments from genomic DNA.
- Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae
- Improved α-Amylase Production by Dephosphorylation Mutation of CreD, an Arrestin-Like Protein Required for Glucose-Induced Endocytosis of Maltose Permease and Carbon Catabolite Derepression in Aspergillusoryzae
- Genetically engineering adenoviral vectors for gene therapy.
- Integration and topology of membrane proteins
- Construction and engineering of large biochemical pathways via DNA assembler
- Direct isolation of specific chromosomal regions and entire genes by TAR cloning.
- Recombinant G protein-coupled receptor expression in Saccharomyces cerevisiae for protein characterization.
- Use of a reporter gene assay in yeast for genetic analysis of DNA-protein interactions.
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