Intracellular distribution of the fluorescent dye nonyl acridine orange responds to the mitochondrial membrane potential: implications for assays of cardiolipin and mitochondrial mass
Explore this paper's citation graph
Summary
It is demonstrated that loading and retention of NAO is dependant upon membrane potential, and that the dye cannot be used as an assay of either cardiolipin or mitochondrial mass in living cells.
- Type
- article
- Published
- 2002-07-01
- Cited by
- 122
- References
- 54
- OpenAlex
- https://openalex.org/W1489968410
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:25487049
Keywords
Cardiolipin, Acridine orange, Mitochondrion, Inner mitochondrial membrane, Phospholipid
References
- The biosynthesis and functional role of cardiolipin.
- The coordinate release of cytochrome c during apoptosis is rapid, complete and kinetically invariant
- Reversible, nonionic, and pH-dependent association of cytochrome c with cardiolipin-phosphatidylcholine liposomes.
- Imaging mitochondrial function in intact cells.
- Cardiolipin provides specificity for targeting of tBid to mitochondria
- Evidence for two distinct acidic phospholipid-binding sites in cytochrome c.
- Cardiolipin requirement for electron transfer in complex I and III of the mitochondrial respiratory chain.
- The Role of a Mitochondrial Pathway in the Induction of Apoptosis by Chemicals Extracted from Diesel Exhaust Particles1
- Preparation of synaptic and nonsynaptic mitochondria from mammalian brain.
- Detection of changes in mitochondrial function during apoptosis by simultaneous staining with multiple fluorescent dyes and correlated multiparameter flow cytometry.
- Specific cardiolipin binding interferes with labeling of sulfhydryl residues in the adenosine diphosphate/adenosine triphosphate carrier protein from beef heart mitochondria.
- 10N-nonyl acridine orange interacts with cardiolipin and allows the quantification of this phospholipid in isolated mitochondria.
- Über hydrophobe Acridinfarbstoffe zur Fluorochromierung von Mitochondrien in lebenden Zellen
- Mitochondrial decay in aging.
- Lack of Mitochondrial Anionic Phospholipids Causes an Inhibition of Translation of Protein Components of the Electron Transport Chain
- Endotoxin pretreatment in vivo increases the mitochondrial respiratory capacity in rat hepatocytes.
- Fluorescent cationic probes of mitochondria. Metrics and mechanism of interaction.
- Screening assays for the mitochondrial permeability transition using a fluorescence multiwell plate reader.
- Human epidermal cells progressively lose their cardiolipins during ageing without change in mitochondrial transmembrane potential.
- Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties.
Cited by
- Binding to and photo-oxidation of cardiolipin by the phthalocyanine photosensitizer Pc 4.
- Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single fluorescent biosensor
- Expression of new prognostic markers, peripheral-type benzodiazepine receptor and carbonic anhydrase IX, in human breast and ovarian carcinoma cell lines.
- Visualization and quantification of mitochondrial dynamics in living animal cells.
- Reduced mitochondrial function but not mitochondrial mass in cerebellar granule cells of homozygous Ca(v) 2.1 mutant mice (leaner mice) at postnatal age 20
- Characterization of SLP-2 function in T lymphocytes
- Oxygen-sensing and Mitochondrial Control of Cell Function
- Osmolyte protection of Escherichia coli and Salmonella enterica against inactivation by acetic acid
- Screening the Human Genome for New Mitochondrial and Longevity Regulators
- Critical role of AMP-activated protein kinase in the balance between mitophagy and mitochondrial biogenesis in MELAS disease.
- Flow cytometric analysis of mitochondria from CA1 and CA3 regions of rat hippocampus reveals differences in permeability transition pore activation
- Mitochondrial biogenesis in mtDNA‐depleted cells involves a Ca2+‐dependent pathway and a reduced mitochondrial protein import
- Modulation of cytochrome c release by mitochondrial redox status and caspase-2
- Loss of mitochondrial thioredoxin reductase delays angiogenic switch and impairs tumour growth
- Screening of effective pharmacological treatments for MELAS syndrome using yeasts, fibroblasts and cybrid models of the disease
- The cytosolic or the mitochondrial glutathione peroxidase‐type tryparedoxin peroxidase is sufficient to protect procyclic Trypanosoma brucei from iron‐mediated mitochondrial damage and lysis
- Structural insight into the interactions between a cationic dye and an anionic surfactant in crystals of 9-aminoacridinium dodecyl sulfate
- Exposure to Heavy Ion Radiation Induces Persistent Oxidative Stress in Mouse Intestine
- Melatonin reduces early changes in intramitochondrial cardiolipin during apoptosis in U937 cell line.
- Glutathione Peroxidase 4 Differentially Regulates the Release of Apoptogenic Proteins from Mitochondria
Related papers
- Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different experimental models.
- Acridine orange staining of smears for demonstration ofMycobacterium tuberculosis
- 10N-nonyl acridine orange interacts with cardiolipin and allows the quantification of this phospholipid in isolated mitochondria.
- Acridine Orange Staining I. In Vitro Derived Cells Lines
- Binding of 10-N-nonyl acridine orange to cardiolipin-deficient yeast cells: implications for assay of cardiolipin.
- Determination of the cardiolipin content of individual mitochondria by capillary electrophoresis with laser‐induced fluorescence detection
- Comparative study of subculture, Gram staining and acridine orange staining for early detection of positive blood cultures.
- Differential staining of bacteria in clinical specimens using acridine orange buffered at low pH.
- Increasing mitochondrial membrane phospholipid content lowers the enzymatic activity of electron transport complexes.
- Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange.