Isolation of RNA.
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Summary
This chapter discusses various methods of isolating RNA, and the efficiency and quality of the nuclei can be ascertained by examination of the cell suspension before and after cell lysis in a phase-contrast microscope.
- Type
- article
- Published
- 1989-01-01
- Cited by
- 75
- References
- 17
- OpenAlex
- https://openalex.org/W974346466
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:31553284
Keywords
RNA, Lysis, Polysome, Cytoplasm, RNA extraction
References
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- The Ribonucleic Acids
- Reaction of diethyl pyrocarbonate with nucleic acid components. Adenosine-containing nucleotides and dinucleoside phosphates.
- Changes in liver-specific compared to common gene transcription during primary culture of mouse hepatocytes
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- Diethyl pyrocarbonate: an examination of its properties in buffered solutions with a new assay technique.
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- Sedimentation characteristics of rapidly labelled RNA from HeLa cells.
- Stimulation of proteinase K action by denaturing agents: application to the isolation of nucleic acids and the degradation of 'masked' proteins.
- The class I major histocompatibility antigen gene activated in a line of SV40-transformed mouse cells is H–2Dd, not Qa/Tla
- Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
- Ribonucleic acid isolated by cesium chloride centrifugation.
- Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes.
- Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning.
- Enzymatic catalysis and the transition state theory of reaction rates: transition state analogs.
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
- A simple, rapid method for the purification of poly A+ RNA.
- Changes in liver-specific compared to common gene transcription during primary culture of mouse hepatocytes.
Cited by
- Molecular investigation of candidate genes involved in the biosynthesis of dinoflagellate paralytic shellfish toxins
- Dexamethasone and nitric oxide synthase gene expression in brain.
- An Overlap between the Control of Programmed Cell Death in Bacillus anthracis and Sporulation
- Differential Glial and Vascular Expression of Endothelins and Their Receptors in Rat Brain after Neurotrauma
- Intracellular Modulation of NMDA Receptor Function by Antipsychotic Drugs
- Mis‐splicing yields circular RNA molecules
- The Pdx1 family is structurally and functionally conserved between Arabidopsis thaliana and Ginkgo biloba
- Quantification of gene expression in single cells
- Participation of p53 protein in the cellular response to DNA damage.
- In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration.
- Characterization of Cyclase Associated Protein homologue, CAP2
- Control of transcription of gal repressor and isorepressor genes in Escherichia coli
- Neuronal adaptation to amphetamine and dopamine: molecular mechanisms of prodynorphin gene regulation in rat striatum.
- Fetuin and Fetuin Messenger RNA in Granulosa Cells of the Rat Ovary1
- Cloning and sequencing of viral integration site in human fibroblasts immortalized by simian virus 40.
- Isolation and characterization of the Candida albicans MOT2 gene.
- FHX.L and FHX.S, two isoforms of the human fork-head factor FHX (FOXJ2) with differential activity.
- Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system.
- Haloperidol-induced Fos expression in striatum is dependent upon transcription factor cyclic AMP response element binding protein.
- Discovery and analysis of antisense oligonucleotide activity in cell culture.
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