Novel methods for proteomics analysis of formalin-fixed, paraffin-embedded tissues (FFPE), and their application for biomarker discovery
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Summary
An optimised method for extraction of full-length proteins from FFPE tissues was developed, and a thorough biomarker discovery study was conducted, analysing human lung neuroendocrine cancer tissues.
- Type
- dissertation
- Published
- 2010-02-08
- Cited by
- 0
- References
- 152
- OpenAlex
- https://openalex.org/W954593143
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:83304457
Keywords
Proteomics, Biomarker discovery, Biology, Biomarker, Pathology
References
- Proteasome-dependent turnover of protein disulfide isomerase in oxidatively stressed cells.
- Direct analysis and MALDI imaging of formalin-fixed, paraffin-embedded tissue sections.
- CE – a multifunctional application for clinical diagnosis
- miRNAs in cancer: approaches, aetiology, diagnostics and therapy.
- Biomarkers and surrogate endpoints: Preferred definitions and conceptual framework
- Detergent addition to tryptic digests and ion mobility separation prior to MS/MS improves peptide yield and protein identification for in situ proteomic investigation of frozen and formalin‐fixed paraffin‐embedded adenocarcinoma tissue sections
- Severe Burn-Induced Endoplasmic Reticulum Stress and Hepatic Damage in Mice
- Metabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis
- Classification of small cell lung cancer and pulmonary carcinoid by gene expression profiles.
- Large-scale analysis of the yeast proteome by multidimensional protein identification technology
- High resolution two-dimensional electrophoresis of proteins.
- Oncomirs — microRNAs with a role in cancer
- S100–annexin complexes – biology of conditional association
- Transcriptional gene expression profiling of small cell lung cancer cells.
- From genomics to proteomics
- Stathmin Expression in Pheochromocytomas, Paragangliomas, and in other Endocrine Tumors
- Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry
- Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues.
- ERp27, a New Non-catalytic Endoplasmic Reticulum-located Human Protein Disulfide Isomerase Family Member, Interacts with ERp57*
- Modeling formalin fixation and antigen retrieval with bovine pancreatic RNase A II. Interrelationship of cross-linking, immunoreactivity, and heat treatment
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