On-line control of glucose feeding in an Escherichia coli fed-batch cultivation expressing a recombinant protein.
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Summary
A new method of controlling the glucose feeding in an Escherichia coli fed-batch cultivation expressing the green fluorescent protein was investigated and showed that the controller could be a powerful tool to create a more secure reproducibility and to generate high product yields in recombinant protein productions.
- Type
- article
- Published
- 2011-01-01
- Cited by
- 0
- References
- 36
- Access
- Open access
- OpenAlex
- https://openalex.org/W309430270
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:8827141
Keywords
Bioprocess, Escherichia coli, Recombinant DNA, Fed-batch culture, Biochemistry
References
- Biology of microorganisms
- Applied Molecular Genetics
- Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations
- A predictive and feedback control algorithm maintains a constant glucose concentration in fed-batch fermentations
- Molecular Biology and Biotechnology
- Recent developments in the monitoring, modeling and control of biological production systems
- Comparison of acetate inhibition on growth of host and recombinantE. coli K12 strains
- A study of the effect of specific growth rate and acetate on recombinant protein production of Escherichia coli JM107
- Avoiding acetate accumulation in Escherichia coli cultures using feedback control of glucose feeding.
- Scale-up of dialysis fermentation for high cell density cultivation of Escherichia coli.
- Soft-sensors for process estimation and inferential control
- Software sensors for fermentation processes
- Green fluorescent protein--a bright idea for the study of bacterial protein localization.
- Data-driven Soft Sensors in the process industry
- Evaluation of software sensors for on-line estimation of culture conditions in an Escherichia coli cultivation expressing a recombinant protein.
- Off-line monitoring of bacterial stress response during recombinant protein production using an optical biosensor.
- Software sensors in bioprocess engineering
- GFP family: structural insights into spectral tuning.
- Green fluorescent protein as a marker for gene expression.
- Overcoming acetate in Escherichia coli recombinant protein fermentations.
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