Construct for high-level expression and low misincorporation of lysine for arginine during expression of pET-encoded eukaryotic proteins in Escherichia coli.
Explore this paper's citation graph
Summary
Competent BL21(DE3)pJY2 cells can be used to suppress lysine misincorporation and achieve high-level expression of pET-encoded target genes without modification of AGA codons in the target gene sequence.
- Type
- article
- Published
- 1999-11-01
- Cited by
- 57
- References
- 0
- Access
- Open access
- OpenAlex
- https://openalex.org/W303090298
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:44820359
Keywords
Gene, Biology, Escherichia coli, Lysine, Transfer RNA
References
No references recorded for this paper.
Cited by
- Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.
- In vitro reconstitution of SCF substrate ubiquitination with purified proteins.
- Structural characterization of membrane proteins by solid-state NMR spectroscopy
- Chemical and genetic strategies for manipulating polyubiquitin chain structure.
- The DNA repair protein Rad23 is a negative regulator of multi-ubiquitin chain assembly
- Controlled synthesis of polyubiquitin chains.
- Cytomegalovirus-induced destruction of MHC class I molecules provides insight into ER-associated protein degradation
- Protein production and purification
- Defining the Ubiquitin and E2-Enzyme Requirements for APC/C-Mediated Degradation of Cyclin B1
- The Production of Improved Tissue-Type Plasminogen Activator in Escherichia coli
- Specific interactions by the N‐terminal arm inhibit self‐association of the AraC dimerization domain
- APC/C-mediated multiple monoubiquitination provides an alternative degradation signal for cyclin B1
- Involvement of the DNA Repair Protein hHR23 inp53Degradation
- Structure and Ubiquitin Binding of the Ubiquitin-interacting Motif*
- The effects of deimination of myelin basic protein on structures formed by its interaction with phosphoinositide-containing lipid monolayers.
- An Arg/Lys-->Gln mutant of recombinant murine myelin basic protein as a mimic of the deiminated form implicated in multiple sclerosis.
- Key Role of Ubc5 and Lysine-63 Polyubiquitination in Viral Activation of IRF3
- Diverse Effects of Pathogenic Mutations of Parkin That Catalyze Multiple Monoubiquitylation in Vitro*
- Glucose Conjugation of Anthranilate by theArabidopsis UGT74F2 Glucosyltransferase Is Required for Tryptophan Mutant Blue Fluorescence*
- Molecular determinants of polyubiquitin linkage selection by an HECT ubiquitin ligase
Related papers
- Successful spread of mcr-1-bearing IncX4 plasmids is associated with variant in replication protein of IncX4 plasmids
- Amino acid requirements of the young turkey: lysine and arginine.
- Effect of excess lysine on utilization of and requirement for arginine by the chick.
- Alterations in lysine transfer RNA during erythroid differentiation of the Friend cell.
- Excess dietary lysine induces hypercholesterolemia in chickens
- Effects of arginine and protein on chicks' responses to dietary lysine.
- Kinetics of chemical modification of arginine and lysine residues in calf thymus histone H1
- The arginine-lysine interaction in the chick.
- LYSINE--ARGININE ANTAGONISM IN THE CHICK.