Construction of plant bacterial artificial chromosome (BAC) libraries: an illustrated guide.
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Summary
This protocol is sufficiently detailed to be of use to both new and experienced investigators and it is hoped that by reducing the obstacles to BAC cloning in plants, it will foster new and accelerated progress in plant genomics.
- Type
- article
- Published
- 2000-01-01
- Cited by
- 163
- References
- 78
- OpenAlex
- https://openalex.org/W184767028
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:82971616
Keywords
Bacterial artificial chromosome, Cloning (programming), Genomics, Biology, Computational biology
References
- Preparation of clone libraries in yeast artificial-chromosome vectors.
- Shotgun Sequencing of the Human Genome
- Locus-specific contig assembly in highly-duplicated genomes, using the BAC-RF method.
- Fluorescence-based sequencing directly from bacterial and P1-derived artificial chromosomes.
- Protection of megabase-sized chromosomal DNA from breakage by DNase activity in plant nuclei.
- Construction and characterization of a maize bacterial artificial chromosome (BAC) library for the inbred line LH132.
- Construction and characterization of a BAC library for the soybean cultivar A3244.
- Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles.
- Construction and characterization of a bovine bacterial artificial chromosome library.
- Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
- A complete BAC-based physical map of the Arabidopsis thaliana genome
- Analysis of chromosome segregation in Saccharomyces cerevisiae.
- Grape (Vitis vinifera L.) BAC Library Construction, Preliminary STC Analysis, and Identification of Clones Associated with Flavonoid and Stilbene Biosynthesis
- Analysis of the quality and utility of random shotgun sequencing at low redundancies.
- A bacterial artificial chromosome library for barley (Hordeum vulgare L.) and the identification of clones containing putative resistance genes
- Construction and characterisation of a large DNA insert library from the D genome of wheat
- Assembly of high-resolution restriction maps based on multiple complete digests of a redundant set of overlapping clones.
- Genome shortcut leads to problems.
- Genetic and physical mapping of xa13, a recessive bacterial blight resistance gene in rice
- A bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene
Cited by
- Preparation of samples for comparative studies of plant chromosomes using in situ hybridization methods.
- Sequence analysis of two alleles reveals that intra-and intergenic recombination played a role in the evolution of the radish fertility restorer (Rfo)
- Adventures in the Enormous: A 1.8 Million Clone BAC Library for the 21.7 Gb Genome of Loblolly Pine
- Preparation of High Molecular Weight (HMW) Genomic DNA from tea Plant (Camellia sinensis) for BAC Library Construction
- Divergence of satellite DNA and interspersion of dispersed repeats in the genome of the wild beet Beta procumbens
- Construction of an Indian Muntjac BAC Library and Production of the Most Highly Dense FISH Map of the Species
- A novel salt-tolerant chitobiosidase discovered by genetic screening of a metagenomic library derived from chitin-amended agricultural soil
- Fine Mapping of Biomass Yield Quantitative Trait Loci in Lolium Perenne L.
- The radish genome and comprehensive gene expression profile of tuberous root formation and development
- Development of tools for the sustainable management of genetics in polyploid Pacific oysters (Crassostrea gigas)
- Analysis of the nuclear proteome of the resurrection plant Xerophyta viscosa (Baker) and its response to dehydration stress
- Complete Genome Sequence of Sporisorium scitamineum and Biotrophic Interaction Transcriptome with Sugarcane
- Transcriptional regulation of taxol™ biosynthesis in taxus cuspidate procambium cells
- Development of genomic resources for cotton (Gossypium hirsutum L.): BAC library construction, preliminary STC analysis, and identification of clones associated with fiber development
- The Glycine max cv. Enrei Genome for Improvement of Japanese Soybean Cultivars
- Construction of a barley bacterial artificial chromosome library suitable for cloning genes for boron tolerance, sodium exclusion and high grain zinc content
- Construction of bacterial artificial chromosome libraries for use in phylogenetic studies.
- Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)
- Genome-level studies on late maturity alpha amylase and boron tolerance in wheat
- The dynamics of LTR retrotransposon accumulation across 25 million years of panicoid grass evolution
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