Improved in situ hybridization: color intensity enhancement procedure for the alkaline phosphatase/Fast Red system.

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Summary

It is found that color intensity can be dramatically increased by adding sodium chloride in the color development solution without secondary antibody amplification, and that the degree of signal augmentation is correlated with sodium chloride concentration.

Type
article
Published
1996-06-01
Cited by
7
References
3
Access
Open access

Keywords

In situ, In situ hybridization, Alkaline phosphatase, Intensity (physics), Chemistry

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