Detection of small non-coding RNAs.
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Summary
This chapter describes optimised RNA extraction methods, which give good yields of shortRNAs, and separation, transfer and hybridisation protocols to study the accumulation of short RNAs.
- Type
- article
- Published
- 2010-01-01
- Cited by
- 3
- References
- 11
- OpenAlex
- https://openalex.org/W88846118
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:9824486
Keywords
RNA, Small nucleolar RNA, Long non-coding RNA, Biology, RNA extraction
References
- Endogenous trans-acting siRNAs regulate the accumulation of Arabidopsis mRNAs.
- Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes.
- Genetic and Functional Diversification of Small RNA Pathways in Plants
- Endogenous siRNAs Derived from a Pair of Natural cis-Antisense Transcripts Regulate Salt Tolerance in Arabidopsis
- The role of small RNAs in abiotic stress
- A simple and efficient method for isolating RNA from pine trees
- Deep sequencing of tomato short RNAs identifies microRNAs targeting genes involved in fruit ripening.
- Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot
- The developmental role of microRNA in plants.
- MicroRNAS and their regulatory roles in plants.
- SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis.
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