An improved method for post-PCR purification for mtDNA sequence analysis.
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Summary
When the yield of purified amplicons, quality of the sequence profile, and ease of assay were evaluated, the use of ExoSAP-IT reagent for post-amplification purification was chosen to replace the filtration method.
- Type
- article
- Published
- 2002-07-01
- Cited by
- 81
- References
- 7
- OpenAlex
- https://openalex.org/W70486235
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:26785600
Keywords
Amplicon, Chromatography, Mitochondrial DNA, Filtration (mathematics), Polymerase chain reaction
References
- Mitochondrial DNA sequence analysis of human skeletal remains: identification of remains from the Vietnam War.
- Mitochondrial DNA sequencing of shed hairs and saliva on robbery caps: sensitivity and matching probabilities.
- Extraction, PCR amplification and sequencing of mitochondrial DNA from human hair shafts.
- Quantitation of polymerase chain reaction products by capillary electrophoresis using laser fluorescence.
- Capillary electrophoresis of polymerase chain reaction-amplified DNA using fluorescence detection with an intercalating dye
- Identifying individuals by sequencing mitochondrial DNA from teeth
- Simulated moving-bed chromatography for continuous separation of two components and its application to bioreactors
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- SeqSharp: A general approach for improving cycle-sequencing that facilitates a robust one-step combined amplification and sequencing method.
- Investigation of Phytophthora species: Phytophthora colocasiae on Taro and Phytophthora Recovered from Streams in Eastern Tennessee
- Long-Range HIV Genotyping Using Viral RNA and Proviral DNA for Analysis of HIV Drug Resistance and HIV Clustering
- A standard bacterial isolate set for research on contemporary dairy spoilage.
- Melanocortin 1 receptor (MC1R) genotype influences erythemal sensitivity to psoralen–ultraviolet A photochemotherapy
- Identification of Intermediate in Evolutionary Model of Enterohemorrhagic Escherichia coli O157
- A vertically-stacked, polymer, microfluidic point mutation analyzer: Rapid, high accuracy detection of low-abundance K-ras mutations
- Dynamics and Timing of in Vivo Mutations at Gag Residue 242 during Primary HIV-1 Subtype C Infection
- Expression line approach to recombinant human epidermal growth factor into the yeast, Pichia pastoris from Huh-7 cell line
- Evolutionary diversification of the gall midge genus Asteromyia (Cecidomyiidae) in a multitrophic ecological context.
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- Mitochondrial DNA Validation in a State Laboratory *,†
- Phylogenetic Relatedness of Circulating HIV-1C Variants in Mochudi, Botswana
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- Biohydroxylation of N,N-dialkylarylamines by the isolated topsoil bacterium Bacillus megaterium
- Genomic characterization of self-incompatibility ribonucleases in the Central Asian pear germplasm and introgression of new alleles from other species of the genus Pyrus
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