Mutation detection using ligase chain reaction in passivated silicon-glass microchips and microchip capillary electrophoresis.
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Summary
A dynamic polymer-based surface passivation method for LCR conducted in oxide-coated silicon-glass microchips, with yields of ligated primers comparable to reactions performed in conventional reaction tubes.
- Type
- article
- Published
- 2004-09-01
- Cited by
- 16
- References
- 32
- Access
- Open access
- OpenAlex
- https://openalex.org/W47515798
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:20125726
Keywords
Capillary electrophoresis, Ligase chain reaction, Polyvinylpyrrolidone, DNA ligase, Passivation
References
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- Microchips: an all-language literature survey including books and patents.
- A single nucleotide polymorphism in the matrix metalloproteinase-1 promoter enhances lung cancer susceptibility.
- Genetic disease detection and DNA amplification using cloned thermostable ligase.
- The Use of Denaturing High-Performance Liquid Chromatography (DHPLC) for the Analysis of Genetic Variations: Impact for Diagnostics and Pharmacogenetics
- PCR as a diagnostic tool for brucellosis.
- Integration of molecular diagnostics with therapeutics: implications for drug discovery and patient care
- Towards dynamic coating of glass microchip chambers for amplifying DNA via the polymerase chain reaction
- Single nucleotide polymorphism genotyping of aldehyde dehydrogenase 2 gene using a single bacterial magnetic particle.
- Blood compatibility of polyethylene oxide surfaces
- Integrated cell isolation and polymerase chain reaction analysis using silicon microfilter chambers.
- Single nucleotide polymorphism (D68D, T to C) in the syntaxin 1A gene correlates to age at onset and insulin requirement in Type II diabetic patients
- Ultrasensitive probing of the protein resistance of PEG surfaces by secondary ion mass spectrometry.
- Polymerase chain reaction in polymeric microchips: DNA amplification in less than 240 seconds.
- The spectrum of cystic fibrosis mutations.
Cited by
- High resolution DNA separations using microchip electrophoresis.
- Fabrication of silicon and glass devices for microfluidic bioanalytical applications
- Serial processing of biological reactions using flow-through microfluidic devices: coupled PCR/LDR for the detection of low-abundant DNA point mutations.
- Real-time analysis of PCR inhibition on microfluidic materials
- Nucleic acid amplification-based techniques for pathogen detection and identification
- Multiplex ligase‐based genotyping methods combined with CE
- Microfluidic Reactor for Sequential Operation of Polymerase Chain Reaction/Ligase Detection Reaction
- Synthesis and self‐assembly of poly(styrene)‐b‐poly(N‐vinylpyrrolidone) amphiphilic diblock copolymers made via a combined ATRP and MADIX approach
- Extraction, amplification and detection of DNA in microfluidic chip-based assays
- DNA mutation detection and analysis using miniaturized microfluidic systems
- Affordable assays for genotyping single nucleotide polymorphisms in insects
- Microchips for Isothermal Amplification of RNA : Development of microsystems for analysis of bacteria, virii and cells
- Detection of enteropathogenic Escherichia coli by microchip capillary electrophoresis.
- Biodegradable polymeric nanostructures in therapeutic applications: opportunities and challenges
- Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications
- Microchip Methods in Diagnostics
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