Practical considerations for large-scale gut microbiome studies
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Summary
It is shown that much can be gained from an optimised transport chain and sample aliquoting before freezing and that other protocols can be useful as long as they preserve the microbial signature of a sample such that relevant conclusions can be drawn regarding the research question, and the obtained data are stable and reproducible over time.
- Type
- article
- Published
- 2017-06-30
- Cited by
- 176
- References
- 82
- Access
- Open access
- OpenAlex
- https://openalex.org/W28830090
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:4965836
Keywords
Geography
References
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- The Human Gut Microbiome: From Association to Modulation.
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- The Accumulating Data to Optimally Predict Obesity Treatment (ADOPT): Recommendations from the Biological Domain
- Comparing Microbiome Sampling Methods in a Wild Mammal: Fecal and Intestinal Samples Record Different Signals of Host Ecology, Evolution
- Measures of reproducibility in sampling and laboratory processing methods in high-throughput microbiome analysis
- A Comparative Analysis of Microbial DNA Preparation Methods for Use With Massive and Branching Coral Growth Forms
- Population-level analysis of Blastocystis subtype prevalence and variation in the human gut microbiota
- Comparison of Fecal Collection Methods for Microbiome and Metabolomics Studies
- Evaluation of DESS as a storage medium for microbial community analysis
- Neurotropic enteroviruses co-opt “fair-weather-friend” commensal gut microbiota to drive host infection and central nervous system disturbances
- Impact of inter- and intra-individual variation, sample storage and sampling fraction on human stool microbial community profiles
- The human microbiome in health and disease: hype or hope
- Effects of the long-term storage of human fecal microbiota samples collected in RNAlater
- A core microbiota of the plant-earthworm interaction conserved across soils
- The gut virome: the ‘missing link’ between gut bacteria and host immunity?
- Issues and current standards of controls in microbiome research
- A role for a leaky gut and the intestinal microbiota in the pathophysiology of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)
- International Cancer Microbiome Consortium consensus statement on the role of the human microbiome in carcinogenesis
- End-to-end assessment of fecal bacteriome analysis: from sample processing to DNA sequencing and bioinformatics results
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