A convenient test for lipase activity in aqueous-based solutions.
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Summary
A convenient and accurate method for the measurement of lipase activity in a uniform aqueous-based substrate solution is proposed and lipase's catalytic performance in this condition followed Michealis-Menten kinetics.
- Type
- article
- Published
- 2015-04-01
- Cited by
- 38
- References
- 29
- OpenAlex
- https://openalex.org/W2078676205
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:31524106
Keywords
Lipase, Candida rugosa, Chemistry, Chromatography, Aqueous solution
References
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- Relationship between sequence conservation and three‐dimensional structure in a large family of esterases, lipases, and related proteins
- Polymer materials for enzyme immobilization and their application in bioreactors.
- Lipase-catalyzed synthesis of isoamyl butyrate. A kinetic study.
- Enzymatic catalysis in nonaqueous solvents.
- Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens
- Covalent immobilization of lipase from Candida rugosa onto poly(acrylonitrile-co-2-hydroxyethyl methacrylate) electrospun fibrous membranes for potential bioreactor application.
- Immobilisation and application of lipases in organic media.
- Assessing a mixture of biosurfactant and enzyme pools in the anaerobic biological treatment of wastewater with a high-fat content
- Chitosan-functionalized poly(methyl methacrylate) particles by spinning disk processing for lipase immobilization.
- Immobilization of lipase onto cellulose ultrafine fiber membrane for oil hydrolysis in high performance bioreactor
- Enzyme catalysis in organic solvents: influence of water content, solvent composition and temperature on Candida rugosa lipase catalyzed transesterification.
- Lipase catalysis in the preparation of 3-(1-amino-3-butenyl)pyridine enantiomers
- A newly discovered bacteriocin from Weissella hellenica D1501 associated with Chinese Dong fermented meat (Nanx Wudl)
- Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases.
- Lipase activity and conformation in neat organic solvents
- Chitosan from Syncephalastrum racemosum Using Sugar Cane Substrates as Inexpensive Carbon Sources
- A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex
- Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: A simple test for the determination of lipase activity in organic media
- Synthesis of sugar fatty acid esters by modified lipase.
Cited by
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- The influence of synthesis conditions on enzymatic activity of enzyme-inorganic hybrid nanoflowers
- Characterization of a secretory hydrolase from Mycobacterium tuberculosis sheds critical insight into host lipid utilization by M. tuberculosis
- Molecular switching system using glycosylphosphatidylinositol to select cells highly expressing recombinant proteins
- Bacterial lipases: A review on purification and characterization.
- Lipase B from Candida antarctica Immobilized on Epoxy-functionalized Hollow Silica Microspheres: Efficient Biocatalysts for Enantiomer Selective Acylation of Alcohols and Amines
- Chemoenzymatic Dynamic Kinetic Resolution of Amines in Fully Continuous-Flow Mode.
- Bacterial enzyme based spectrophotometric determination of phthalate esters in drinking water stored in PET bottles
- Effect of high-pressure pre-treatment on the enzymatic activity related to quality loss in frozen hake
- Easy-cleaning surfaces functionalized with an interface-binding recombinant lipase.
- The occurrence, enzymatic production, and application of ethyl butanoate, an important flavor constituent
- Selecting cells expressing high levels of recombinant proteins using the GPI-anchored protein with selenocysteine system.
- Lipolytic and antimicrobial activities of Pseudomonas strains isolated from soils in Phetchaburi Province, Thailand
- Immobilization of Candida rugosa lipase for resolution of racimic ibuprofen
- Inhibition of Lipase by Orlistat: Kinetics Combined with In Silico Approaches to Visualize Interactions
- Characterization and application of natural and recombinant butelase-1 to improve industrial enzymes by end-to-end circularization
- Virus-templated magnetic composite hydrogels for surface immobilization of mimic-free-lipase.
- An Electrospun Sandwich-Type Lipase-Membrane Bioreactor for Hydrolysis at Macroscopic Oil-Water Interfaces.
- Application of Hierarchical Clustering to Analyze Solvent-Accessible Surface Area Patterns in Amycolatopsis lipases
- Microbial Enzymes: Roles and Applications in Industries
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