Structure-based design of novel chemical modification of the 3'-overhang for optimization of short interfering RNA performance.
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Summary
This work designed and synthesized a set of siRNAs containing modified two-nucleotide 3'-overhangs with the aim of strengthening the interaction between the 3'-end of the siRNA strand and the PAZ domain of Ago2, and helps to develop the computer modeling method for rational siRNA design.
- Type
- article
- Published
- 2015-01-30
- Cited by
- 21
- References
- 30
- OpenAlex
- https://openalex.org/W25635512
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:41187364
Keywords
Saturable absorption, Materials science, Carbon nanotube, Fiber laser, Doping
References
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- Functional siRNAs and miRNAs exhibit strand bias.
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- Synthesis of nuclease-resistant siRNAs possessing universal overhangs.
Cited by
- Synthesis of small interfering RNAs containing acetal-type nucleoside analogs at their 3'-ends and analysis of their silencing activity and their ability to bind to the Argonaute2 PAZ domain.
- Progress and Prospects of Anti-HBV Gene Therapy Development
- siRNA and RNAi optimization
- Guide Strand 3′-End Modifications Regulate siRNA Specificity
- Synthesis of Nucleic Acid Mimics and Their Application in Nucleic Acid-based Medicine.
- Probing the Binding Interactions between Chemically Modified siRNAs and Human Argonaute 2 Using Microsecond Molecular Dynamics Simulations
- Therapeutic potential of chemically modified siRNA: Recent trends
- Introduction of 2-O-benzyl abasic nucleosides to the 3'-overhang regions of siRNAs greatly improves nuclease resistance.
- Exploring PAZ/3′-overhang interaction to improve siRNA specificity. A combined experimental and modeling study
- Synthesis of RNA Bearing C5-polyamine Modified Pyrimidine Nucleosides at the 3´-end by T4 RNA Ligase
- Synthesis and characterization of small interfering RNAs with haloalkyl groups at their 3'-dangling ends.
- Systemic Delivery of Small Interfering RNA Therapeutics: Obstacles and Advances
- Developments in siRNA Modification and Ligand Conjugated Delivery To Enhance RNA Interference Ability
- Sulfonamide antibiotics inhibit RNAi by binding to human Argonaute protein 2 PAZ.
- Fluorescence Lifetime Imaging Microscopy (FLIM) of Intracellular Transport by Means of Doubly Labelled siRNA Architectures
- How to Computationally Stack the Deck for Hit-to-Lead Generation: In Silico Molecular Interaction Energy Profiling for de Novo siRNA Guide Strand Surrogate Selection
- RNA Interference Therapeutics for Chronic Hepatitis B: Progress, Challenges, and Future Prospects
- Advances in structural-guided modifications of siRNA.
- Improving the potency prediction for chemically modified siRNAs through insights from molecular modeling of individual sequence positions
- 3'-C-Extended TNA: De Novo Synthesis, Enhanced Exonuclease Resistance, and Functional siRNA 3'-Overhang Modifications Without Compromising Gene Silencing.
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