Validation of macromolecular flexibility in solution by small-angle X-ray scattering (SAXS)
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Summary
This review addresses theoretical and practical concepts, concerns, and considerations for using techniques in conjunction with computational methods to productively combine solution-scattering data with high-resolution structures to identify macromolecular flexibility from SAXS data.
- Type
- review
- Published
- 2012-05-26
- Cited by
- 122
- References
- 81
- Access
- Open access
- OpenAlex
- https://openalex.org/W2018816634
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:6706881
Keywords
Small-angle X-ray scattering, Macromolecule, Flexibility (engineering), Scattering, Molecular dynamics
References
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- Self-association and domain rearrangements between complement C3 and C3u provide insight into the activation mechanism of C3.
- Structural characterization of intramolecular Hg(2+) transfer between flexibly linked domains of mercuric ion reductase.
- SAXS ensemble refinement of ESCRT-III CHMP3 conformational transitions
- Determination of the regularization parameter in indirect-transform methods using perceptual criteria
- The immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR adopt an unusual horseshoe-like conformation
- Semi-rigid solution structures of heparin by constrained X-ray scattering modelling: new insight into heparin-protein complexes.
- Refinement of multidomain protein structures by combination of solution small-angle X-ray scattering and NMR data.
- X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
- Characterizing Flexible and Instrinsically Unstructured Biological Macromolecules by SAS using the Porod-Debye Law
- Human DNA ligase I completely encircles and partially unwinds nicked DNA
- Activation mechanisms of transcriptional regulator CooA revealed by small-angle X-ray scattering.
- Structural dynamics in DNA damage signaling and repair
- Structure determinations of human and chimaeric antibodies by solution scattering and constrained molecular modelling.
- Improved Fitting of Solution X-ray Scattering Data to Macromolecular Structures and Structural Ensembles by Explicit Water Modeling
- Degradation of Cellulose Substrates by Cellulosome Chimeras
- Structural Mechanism of Abscisic Acid Binding and Signaling by Dimeric PYR1
- XLF regulates filament architecture of the XRCC4·Ligase IV complex
Cited by
- Architecture of DNA Bound RAR heterodimers.
- A Single Amino-Acid Substitution Allows Endo-Polygalacturonase of Fusarium verticillioides to Acquire Recognition by PGIP2 from Phaseolus vulgaris
- Prion Protein-Antibody Complexes Characterized by Chromatography-Coupled Small-Angle X-Ray Scattering.
- Preformed Soluble Chemoreceptor Trimers That Mimic Cellular Assembly States and Activate CheA Autophosphorylation
- Modeling Assemblies and Interactions at the Replication Fork: Sliding Clamps and Clamp Interacting Enzymes
- Crystallographic and solution studies of NAD(+)- and NADH-bound alkylhydroperoxide reductase subunit F (AhpF) from Escherichia coli provide insight into sequential enzymatic steps.
- 112 Structurally distinct ubiquitin- and SUMO-modified PCNA: implications for their distinct roles in the DNA damage response
- Conformational Transitions that Enable Histidine Kinase Autophosphorylation and Receptor Array Integration
- An introduction to biomolecular simulations and docking
- Atypical Response Regulator ChxR from Chlamydia trachomatis Is Structurally Poised for DNA Binding
- Time-resolved studies of dynamic biomolecules using small angle X-ray scattering.
- All-atom ensemble modeling to analyze small angle X-ray scattering of glycosylated proteins
- Accurate SAXS profile computation and its assessment by contrast variation experiments.
- Promotion of Enzyme Flexibility by Dephosphorylation and Coupling to the Catalytic Mechanism of a Phosphohexomutase*
- Small-Angle X-Ray Scattering for the Discerning Macromolecular Crystallographer
- Domain mobility as probed by small-angle X-ray scattering may account for substrate access to the active site of two copper-dependent amine oxidases.
- Determination of the GH3.12 protein conformation through HPLC-integrated SAXS measurements combined with X-ray crystallography.
- The Structure of Human Apolipoprotein A-IV as Revealed by Stable Isotope-assisted Cross-linking, Molecular Dynamics, and Small Angle X-ray Scattering*
- Review of the fundamental theories behind small angle X-ray scattering, molecular dynamics simulations, and relevant integrated application
- Structural Insights into Functional Overlapping and Differentiation among Myosin V Motors*
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