Side-by-Side Comparison of Five Commercial Media Systems in a Mouse Model: Suboptimal In Vitro Culture Interferes with Imprint Maintenance1
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Summary
Comparing side-by-side comparisons of five commercial embryo culture systems shows that embryo culture in all commercial media systems resulted in imprinted methylation loss compared to in vivo-derived embryos, although some media systems were able to maintain imprintedmethylation levels more similar to those of in vitro embryos in comparison to embryos cultured in Whitten medium.
- Type
- dissertation
- Published
- 2010-12-01
- Cited by
- 154
- References
- 69
- Access
- Open access
- OpenAlex
- https://openalex.org/W20702853
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:24164619
Keywords
Humanities, Multicast, Computer science, Art, Computer network
References
- Development of ovine embryos in synthetic oviductal fluid containing amino acids at oviductal fluid concentrations.
- In utero programming of cardiovascular disease.
- Culture of preimplantation embryos.
- Guide to techniques in mouse development
- On Information and Sufficiency
- Biometry: The Principles and Practice of Statistics in Biological Research
- Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid.
- Viability and growth of mouse embryos after in vitro culture and fusion.
- Comparison of two media for sequential culture after IVF and ICSI shows no differences in pregnancy rates: a randomized trial.
- A 5' 2-kilobase-pair region of the imprinted mouse H19 gene exhibits exclusive paternal methylation throughout development
- The murine H19 gene is activated during embryonic stem cell differentiation in vitro and at the time of implantation in the developing embryo.
- Temporal and spatial regulation of H19 imprinting in normal and uniparental mouse embryos.
- In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system.
- A randomized control comparison study of culture media (HTF versus P1) for human in vitro fertilization.
- Culture of viable human blastocysts in defined sequential serum-free media.
- Beckwith-Wiedemann syndrome and IVF: a case-control study.
- Mammalian embryo culture in the absence of serum or somatic cell support
- Maintenance of Paternal Methylation and Repression of the Imprinted H19 Gene Requires MBD3
- Association between Beckwith-Wiedemann syndrome and assisted reproductive technology: a case series of 19 patients.
- Pyruvate and glucose uptake by mouse ova and preimplantation embryos.
Cited by
- Alteration of DNA demethylation dynamics by in vitro culture conditions in rabbit pre-implantation embryos
- Embryo culture and epigenetics.
- Epigenetic effects of methoxychlor and vinclozolin on male gametes.
- Maternal Control of Genomic Imprinting: Effects of Infertility and Ovarian Stimulation in a Mouse Model
- Epigenetic regulation of genomic imprinting from germ line to preimplantation
- Improving embryo quality in assisted reproduction
- Morphological assessment on day 4 and its prognostic power in selecting viable embryos for transfer
- Quality control of embryo development.
- Advances in embryo selection methods
- Variability in protein quality used for embryo culture: embryotoxicity of the stabilizer octanoic acid.
- Altered imprinted gene expression and methylation patterns in mid-gestation aborted cloned porcine fetuses and placentas
- The interfering effects of superovulation and vitrification upon some important epigenetic biomarkers in mouse blastocyst.
- The assisted reproductive technology laboratory: toward evidence-based practice?
- Health outcomes of children born after IVF/ICSI: a review of current expert opinion and literature.
- A Simple Approach for COnsumption and RElease (CORE) Analysis of Metabolic Activity in Single Mammalian Embryos
- Blastomere biopsy influences epigenetic reprogramming during early embryo development, which impacts neural development and function in resulting mice
- Effect of embryo freezing on perinatal outcome after assisted reproduction techniques: lessons from the Latin American Registry of Assisted Reproduction.
- Embryo Manipulation and Imprinting
- Does vitrification alter the methylation pattern of OCT4 promoter in rabbit late blastocyst?
- Long-term and transgenerational effects of in vitro culture on mouse embryos.
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