Operator-assisted harvesting of protein crystals using a universal micromanipulation robot
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Summary
The prototype of a universal micromanipulation robot for crystal harvesting is presented, and a robotically harvested trypsin crystal yields a high-resolution structure demonstrating the feasibility of robotic protein crystal harvesting.
- Type
- article
- Published
- 2007-05-15
- Cited by
- 25
- References
- 34
- Access
- Open access
- OpenAlex
- https://openalex.org/W19461845
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:6249279
Keywords
Persistence (discontinuity), Mythology, Media studies, Sociology, Library science
References
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- Trypsin Revisited
- Phenol stabilizes more helix in a new symmetrical zinc insulin hexamer
- ICM—A new method for protein modeling and design: Applications to docking and structure prediction from the distorted native conformation
- Macromolecular cryocrystallography--methods for cooling and mounting protein crystals at cryogenic temperatures.
- A fully integrated protein crystallization platform for small-molecule drug discovery.
- Raster3D: photorealistic molecular graphics.
- Self-consistent assignment of asparagine and glutamine amide rotamers in protein crystal structures.
- Errors in protein structures
- Towards an understanding of radiation damage in cryocooled macromolecular crystals.
- Accurate Bond and Angle Parameters for X-ray Protein Structure Refinement
- XtalView/Xfit--A versatile program for manipulating atomic coordinates and electron density.
- The crystal structures of the complexes between bovine beta-trypsin and ten P1 variants of BPTI.
- High-throughput crystallography at an affordable cost: the TB Structural Genomics Consortium Crystallization Facility.
- Trypsin as a model for high resolution X-ray diffraction in proteins.
- Structure
Cited by
- To automate or not to automate: this is the question
- Automated robotic harvesting of protein crystals—addressing a critical bottleneck or instrumentation overkill?
- Semi‐automated protein crystal mounting device for the sulfur single‐wavelength anomalous diffraction method
- Development of an alternative approach to protein crystallization
- A robotic arm as a simple sample changer for a diffractometer with very low component costs
- Automation and Experience of Controlled Crystal Dehydration: Results from the European Synchrotron HC1 Collaboration
- Direct cryocooling of naked crystals: are cryoprotection agents always necessary?
- A historical perspective on protein crystallization from 1840 to the present day
- Approaches to automated protein crystal harvesting.
- Non-contact manipulation for automated protein crystal harvesting using a rolling microrobot
- Lessons from high-throughput protein crystallization screening: 10 years of practical experience
- Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes
- The solvent component of macromolecular crystals
- Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening
- First experiences with semi-autonomous robotic harvesting of protein crystals
- The use of workflows in the design and implementation of complex experiments in macromolecular crystallography
- Automated harvesting and processing of protein crystals through laser photoablation
- The Role of Small Molecule Additives and Chemical Modification in Protein Crystallization
- CrystalDirect™: a novel approach for automated crystal harvesting based on photoablation of thin films.
- Analysis of the Quality of Macromolecular Structures
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