Alkaline liquid chromatography/electrospray ionization skimmer collision-induced dissociation mass spectrometry for phosphopeptide screening.
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Summary
The use of alkaline conditions together with a previously described method for selective and sensitive detection of phosphopeptide ions combining high-performance capillary liquid chromatography (LC) and electrospray ionization mass spectrometry (ESI-MS) is investigated.
- Type
- article
- Published
- 2001-12-15
- Cited by
- 24
- References
- 24
- OpenAlex
- https://openalex.org/W11746899
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:7980145
Keywords
Variety (cybernetics), Competence (human resources), Computer science, Process (computing), Mathematics education
References
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- Parent ion scans of unseparated peptide mixtures.
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- Pathophysiology and pharmacological treatment of insulin resistance.
- Phospho‐proteomics: Evaluation of the use of enzymatic de‐phosphorylation and differential mass spectrometric peptide mass mapping for site specific phosphorylation assignment in proteins separated by gel electrophoresis
- Analytical properties of the nanoelectrospray ion source.
- Mapping of phosphorylation sites of gel-isolated proteins by nanoelectrospray tandem mass spectrometry: potentials and limitations.
- Signaling—2000 and Beyond
- Disparity between solution‐phase equilibria and charge state distributions in positive‐ion electrospray mass spectrometry
- Electrospray tandem mass spectrometric studies of phosphopeptides and phosphopeptide analogues.
- Selective, sensitive, and rapid phosphopeptide identification in enzymatic digests using ESI-FTICR-MS with infrared multiphoton dissociation.
- Quantitative in vitro kinase reaction as a guide for phosphoprotein analysis by mass spectrometry.
- Selective detection and sequencing of phosphopeptides at the femtomole level by mass spectrometry.
- A multidimensional electrospray MS-based approach to phosphopeptide mapping.
- Chromatographic and mass spectrometric methods for the identification of phosphorylation sites in phosphoproteins.
- Protein-protein interactions define specificity in signal transduction.
Cited by
- Gas-Phase Acidities of Phosphorylated Amino Acids.
- Synthesis and Characterisation of a Multiphosphorylated Phosphophoryn Repeat Motif; H-[Asp-(Ser(P))2]3-Asp-OH
- Separation, detection, and identification of peptides by ion-pair reversed-phase high-performance liquid chromatography-electrospray ionization mass spectrometry at high and low pH.
- Alkaline conditions in hydrophilic interaction liquid chromatography for intracellular metabolite quantification using tandem mass spectrometry.
- Microcapillary liquid chromatography/tandem mass spectrometry using alkaline pH mobile phases and positive ion detection.
- Metal ion-mobilizing additives for comprehensive detection of femtomole amounts of phosphopeptides by reversed phase LC-MS
- Analysis of microcystins by capillary high performance liquid chromatography using a polymethacrylate-based monolithic column.
- Stainless steel electrospray probe: a dead end for phosphorylated organic compounds?
- Identification of an in vitro insulin receptor substrate-1 phosphorylation site by negative-ion μLC/ES-API-CID-MS hybrid scan technique
- Negative ion fragmentations of deprotonated peptides containing post-translational modifications: diphosphorylated systems containing Ser, Thr and Tyr. A characteristic phosphate/phosphate cyclisation. A joint experimental and theoretical study.
- Characteristic negative ion fragmentations of deprotonated peptides containing post-translational modifications: mono-phosphorylated Ser, Thr and Tyr. A joint experimental and theoretical study.
- Detection of tyrosine phosphorylated peptides via skimmer collision-induced dissociation/ion trap mass spectrometry.
- Skimmer CID‐ion trap mass spectrometry of phosphotyrosine‐containing peptides
- High sequence-coverage detection of proteolytic peptides using a bis(terpyridine)ruthenium(II) complex.
- Die Dynamik von Assemblierungsprozessen des photosynthetischen Apparates der Grünalge Chlamydomonas reinhardtii
- Characterization and identification of protein posttranslational modifications using protein enrichment and mass spectrometry
- Analysis of protein phosphorylation sites using affinity enrichment and mass spectrometry.
- Proteome analysis of posttranslational modifications.
- [Analysis of contribution of protein phosphorylation in the development of the diseases].
- Development of sensitive proteomic approaches for protein tyrosine phosphorylation detection.
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