Stereospecific assignment of β-methylene protons in larger proteins using 3D15N-separated Hartmann-Hahn and13C-separated rotating frame Overhauser spectroscopy

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Summary

It is demonstrated that reliable information on the intraresidue CxH−CβH distances, free of systematic errors arising from spin diffusion, can be obtained from a 3D13C-separated1H−1H rotating frame Overhauser effect 1H−13C multiple quantum coherence (ROESY-HMQC) spectrum.

Type
article
Published
1991-05-01
Cited by
84
References
47

Keywords

Iconography, Art, Art history

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